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Detection of a Geographically Diverse Malarial Biomarker via Multi-Epitope Targeted Screening

Liang, JingXin and Nag, Arundhati and Das, Samir and Bunck, David and McCarthy, Amy and Mishra, Anvita and Heath, John E. and Villalonga, Belen and Heath, James R. (2016) Detection of a Geographically Diverse Malarial Biomarker via Multi-Epitope Targeted Screening. Biophysical Journal, 110 (3). 519A. ISSN 0006-3495. doi:10.1016/j.bpj.2015.11.2774.

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We report on the development of high affinity macrocyclic peptides as ligands for the capture and detection of PfHRP2, a protein without a defined tertiary structure. PfHRP2 is a key biomarker of the fatal falciparum strain of malaria. This intrinsically disordered protein has repeated epitopes that have inconstant occurrence over geographic regions. Thus, current antibody based diagnostic tests that target a single epitope do not account for the sequence diversity of PfHRP2 and exhibit variable performance. Ligands selected via high throughput epitope-targeted in situ click screening with combinatorial macrocylic peptide libraries yield binders with high affinity and selectivity for their protein targets. These macrocyclic peptide binders offer greater biochemical and thermal stability than antibodies. We provide a general strategy for the amplification of sensitivity in disease detection through the development of ligands that target multiple epitopes in a single and unstructured protein biomarker. We apply our macrocyclic peptide ligands towards the development of an antibody free diagnostic test for PfHRP2.

Item Type:Article
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URLURL TypeDescription
Liang, JingXin0000-0001-6600-8409
Bunck, David0000-0002-6246-9846
Heath, James R.0000-0001-5356-4385
Additional Information:© 2016 Biophysical Society. Published by Elsevier Inc.
Issue or Number:3
Record Number:CaltechAUTHORS:20160606-134847419
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Official Citation:JingXin Liang, Arundhati Nag, Samir Das, David Bunck, Amy McCarthy, Anvita Mishra, John E. Heath, Belen Villalonga, James R. Heath, Detection of a Geographically Diverse Malarial Biomarker via Multi-Epitope Targeted Screening, Biophysical Journal, Volume 110, Issue 3, Supplement 1, 16 February 2016, Page 519a, ISSN 0006-3495, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67692
Deposited By: Tony Diaz
Deposited On:06 Jun 2016 21:09
Last Modified:11 Nov 2021 03:53

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