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Mutants of Sindbis Virus I. Isolation and Partial Characterization of 89 New Temperature-Sensitive Mutants

Strauss, Ellen G. and Lenches, Edith M. and Strauss, James H. (1976) Mutants of Sindbis Virus I. Isolation and Partial Characterization of 89 New Temperature-Sensitive Mutants. Virology, 74 (1). pp. 154-168. ISSN 0042-6822. doi:10.1016/0042-6822(76)90137-9.

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More than 100 new temperature-sensitive mutants of Sindbis virus have been isolated, following mutagenesis with nitrous acid, N-methyl-N’-nitro-N-nitrosoguanidine, Sazacytidine, and 5-fluorouridine. Thirty-six of these mutants synthesize at least 60% as much RNA at the nonpermissive temperature as does the parental strain and are designated RNA+; 23 mutants synthesize between 10 and 60% as much RNA as the parental strain at 40° and are designated RNA±; 30 mutants make less than 10% as much RNA at 40° and are called RNA-. The remaining mutants have not been tested for RNA incorporation. The thermal stability at 56° of most of the mutant particles has been examined. The majority of the RNA+ mutants is more sensitive to heating at 56° than the parental HR strain, and RNA+ mutations appear to reside primarily in genes coding for the structural proteins. Approximately 20% of either RNA± or RNA- mutants are thermosensitive, and these mutations thus appear to reside primarily in genes coding for the nonstructural proteins. Complementation assays have been performed with a number of these mutants and with those of Burge and Pfefferkorn (1966a, b). The existence of three complementation groups among the RNA+ mutants, which appear to encode the three major structural proteins, has been confirmed; no new complementation groups among RNA+ mutants have been identified. A total of four complementation groups has been identified among the RNA- mutants. Thus, Sindbis virus contains at least seven complementation groups.

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Additional Information:© 1976 Academic Press, Inc. Accepted May 24, 1976. This work was supported by Grant No. AI 10793 from the U. S. Public Health Service and by a grant from the Research Corporation.
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NIHAI 10793
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Record Number:CaltechAUTHORS:20160606-163953006
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Official Citation:Ellen G. Strauss, Edith M. Lenches, James H. Strauss, Mutants of Sindbis virus, Virology, Volume 74, Issue 1, 1976, Pages 154-168, ISSN 0042-6822, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67706
Deposited By: Donna Wrublewski
Deposited On:06 Jun 2016 23:58
Last Modified:11 Nov 2021 03:53

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