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Infection of Protoplasts of Escherichia coli by Subviral Particles of Bacteriophage φX174

Guthrie, George D. and Sinsheimer, Robert L. (1960) Infection of Protoplasts of Escherichia coli by Subviral Particles of Bacteriophage φX174. Journal of Molecular Biology, 2 (5). pp. 297-305. ISSN 0022-2836.

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Infection of protoplasts of various strains of Escherichia coli may be obtained with purified DNA preparations from bacteriophage φX174 made by two distinctly different methods. Infection of such protoplasts can also be obtained with nucleoprotein particles made from φX174 by heat treatment. None of the particles, infective to protoplasts, are infective to whole cells and all are infective to protoplasts of strains of bacteria usually resistant to whole φX174 virus. The protoplast-infective agents are in all cases destroyed by enzymatic treatment with DNase. The infective DNA is not inactivated by treatment with enzymatic amounts of trypsin but is by treatment with stoichiometric amounts, suggesting the formation of an inactive DNA-trypsin complex. Studies of the properties of protoplast infection indicate certain general characteristics which distinguish it from the more familiar virus infection of whole bacterial cells.

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Additional Information:© 1960 Elsevier Ltd. Received 11 July 1960. It is a pleasure to acknowledge the capable technical assistance of Miss Faith Poole. Several talks with Dr. Dean Fraser, Indiana University, about the preparation and use of protoplasts were of considerable value in developing the techniques for this research. Dr. Wolfgang Mundry's contributions in numerous discussions are gratefully acknowledged. This research has been supported by U.S. Public Health Service grants C-3441 and 2G86. One of us (GDG) has been supported by a U.S. Public Health Predoctoral Fellowship (No.10,078) during most of this work.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)C-3441
U.S. Public Health Service (USPHS)2G86
U.S. Public Health Service (USPHS) Predoctoral Fellowship10,078
Issue or Number:5
Record Number:CaltechAUTHORS:20160610-090908963
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Official Citation:George D. Guthrie, Robert L. Sinsheimer, Infection of Protoplasts of Escherichia coli by Subviral Particles of Bacteriophage φX174, Journal of Molecular Biology, Volume 2, Issue 5, 1960, Pages 297-305, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67815
Deposited By: Ruth Sustaita
Deposited On:10 Jun 2016 16:19
Last Modified:03 Oct 2019 10:08

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