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The Structure of the DNA of Bacteriophage φX174: I. The Action of Exopolynucleotidases

Fiers, Walter and Sinsheimer, Robert L. (1962) The Structure of the DNA of Bacteriophage φX174: I. The Action of Exopolynucleotidases. Journal of Molecular Biology, 5 (4). pp. 408-419. ISSN 0022-2836. doi:10.1016/S0022-2836(62)80029-1. https://resolver.caltech.edu/CaltechAUTHORS:20160610-104406814

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Abstract

A φX-DNA preparation cannot be inactivated, and can be hydrolysed only to a small extent, by E. coli phosphodiesterase. This result is a consequence of the unavailability of a free 3′-OH terminus. Studies of the action of spleen phosphodiesterase upon φX DNA similarly indicate the absence of a free 5′-0H terminus. Inactivation of φX. DNA by spleen or venom phosphodiesterase is exclusively a consequence of endonuclease activities present in these enzyme preparations. Pre-treatment of φX DNA with bacterial Phosphomonoesterase does not increase its susceptibility to the action of E. coli or spleen phosphodiesterase other than by the production of fragments as a consequence of contaminating endonuclease action.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0022-2836(62)80029-1DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283662800291PublisherArticle
Additional Information:© 1962 Elsevier Ltd. Received 20 June 1962. This research was supported by U.S. Public Health Service grant RG6965. One of the authors (W. F.) is a Rockefeller Foundation Fellow; “Chargé de Recherches du Fonds National Belge de la Recherche Scientifique”.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
Rockefeller FoundationUNSPECIFIED
Chargé de Recherches du Fonds National Belge de la Recherche ScientifiqueUNSPECIFIED
Issue or Number:4
DOI:10.1016/S0022-2836(62)80029-1
Record Number:CaltechAUTHORS:20160610-104406814
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160610-104406814
Official Citation:Walter Fiers, Robert L. Sinsheimer, The structure of the DNA of bacteriophage φX174, Journal of Molecular Biology, Volume 5, Issue 4, 1962, Pages 408-419, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(62)80029-1. (http://www.sciencedirect.com/science/article/pii/S0022283662800291)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67829
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Jun 2016 18:11
Last Modified:11 Nov 2021 03:55

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