Fiers, Walter and Sinsheimer, Robert L. (1962) The Structure of the DNA of Bacteriophage φX174: I. The Action of Exopolynucleotidases. Journal of Molecular Biology, 5 (4). pp. 408-419. ISSN 0022-2836. doi:10.1016/S0022-2836(62)80029-1. https://resolver.caltech.edu/CaltechAUTHORS:20160610-104406814
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Abstract
A φX-DNA preparation cannot be inactivated, and can be hydrolysed only to a small extent, by E. coli phosphodiesterase. This result is a consequence of the unavailability of a free 3′-OH terminus. Studies of the action of spleen phosphodiesterase upon φX DNA similarly indicate the absence of a free 5′-0H terminus. Inactivation of φX. DNA by spleen or venom phosphodiesterase is exclusively a consequence of endonuclease activities present in these enzyme preparations. Pre-treatment of φX DNA with bacterial Phosphomonoesterase does not increase its susceptibility to the action of E. coli or spleen phosphodiesterase other than by the production of fragments as a consequence of contaminating endonuclease action.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1962 Elsevier Ltd. Received 20 June 1962. This research was supported by U.S. Public Health Service grant RG6965. One of the authors (W. F.) is a Rockefeller Foundation Fellow; “Chargé de Recherches du Fonds National Belge de la Recherche Scientifique”. | |||||||||
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| Issue or Number: | 4 | |||||||||
| DOI: | 10.1016/S0022-2836(62)80029-1 | |||||||||
| Record Number: | CaltechAUTHORS:20160610-104406814 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160610-104406814 | |||||||||
| Official Citation: | Walter Fiers, Robert L. Sinsheimer, The structure of the DNA of bacteriophage φX174, Journal of Molecular Biology, Volume 5, Issue 4, 1962, Pages 408-419, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(62)80029-1. (http://www.sciencedirect.com/science/article/pii/S0022283662800291) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 67829 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 10 Jun 2016 18:11 | |||||||||
| Last Modified: | 11 Nov 2021 03:55 |
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