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The Structure of the DNA of Bacteriophage φX174: I. The Action of Exopolynucleotidases

Fiers, Walter and Sinsheimer, Robert L. (1962) The Structure of the DNA of Bacteriophage φX174: I. The Action of Exopolynucleotidases. Journal of Molecular Biology, 5 (4). pp. 408-419. ISSN 0022-2836. doi:10.1016/S0022-2836(62)80029-1.

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A φX-DNA preparation cannot be inactivated, and can be hydrolysed only to a small extent, by E. coli phosphodiesterase. This result is a consequence of the unavailability of a free 3′-OH terminus. Studies of the action of spleen phosphodiesterase upon φX DNA similarly indicate the absence of a free 5′-0H terminus. Inactivation of φX. DNA by spleen or venom phosphodiesterase is exclusively a consequence of endonuclease activities present in these enzyme preparations. Pre-treatment of φX DNA with bacterial Phosphomonoesterase does not increase its susceptibility to the action of E. coli or spleen phosphodiesterase other than by the production of fragments as a consequence of contaminating endonuclease action.

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Additional Information:© 1962 Elsevier Ltd. Received 20 June 1962. This research was supported by U.S. Public Health Service grant RG6965. One of the authors (W. F.) is a Rockefeller Foundation Fellow; “Chargé de Recherches du Fonds National Belge de la Recherche Scientifique”.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
Rockefeller FoundationUNSPECIFIED
Chargé de Recherches du Fonds National Belge de la Recherche ScientifiqueUNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20160610-104406814
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Official Citation:Walter Fiers, Robert L. Sinsheimer, The structure of the DNA of bacteriophage φX174, Journal of Molecular Biology, Volume 5, Issue 4, 1962, Pages 408-419, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67829
Deposited By: Ruth Sustaita
Deposited On:10 Jun 2016 18:11
Last Modified:11 Nov 2021 03:55

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