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Translation of Sindbis Virus 26 S RNA and 49 S RNA in Lysates of Rabbit Reticulocytes

Simmons, Daniel T. and Strauss, James H. (1974) Translation of Sindbis Virus 26 S RNA and 49 S RNA in Lysates of Rabbit Reticulocytes. Journal of Molecular Biology, 86 (2). pp. 397-409. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160613-145751806

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Abstract

Sindbis virus-specifc polypeptides were synthesized in lysates of rabbit reticulocytes in response to added 26 S or 49 S RNA. Sindbis 26 S RNA was translated into as many as three polypeptides which co-migrate in acrylamide gels with proteins found in infected cells. Wild type 26 S RNA was translated primarily into two polypeptides, which appear to be the Sindbis nucleocapsid protein (mol. wt 30,000) and the precursor of the two glycoproteins of the virion (mol. wt 100,000). A larger polypeptide (mol. wt 130,000) was synthesized in response to ts2 26 S RNA, a species of RNA which was isolated from cells infected with the ts2 mutant of Sindbis virus. This large polypeptide is apparently the protein which accumulates in cells infected with the mutant virus and which is thought to be a precursor of all three viral structural proteins. These results support the hypothesis that 26 S RNA is the messenger for the three structural proteins of the virion and that the RNA codes for one large polypeptide precursor. The precursor may then be cleaved at a specific site to yield the nucleocapsid protein and a second polypeptide which, in infected cells, is cleaved in a series of steps to yield the two glycoproteins of the virion. Sindbis 49 S RNA was translated into eight or nine polypeptides ranging from 60,000 to 180,000 molecular weights. The viral structural proteins, as such, were not synthesized in response to the added 49 S RNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(74)90027-8DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283674900278PublisherArticle
Additional Information:© 1974 Elsevier Ltd. Received 21 January 1974, and in revised form 13 March 1974. We acknowledge the expert technical assistance of Edith M. Lenches and Sharman E. S. Christoph for preparing the cultures of chick embryo fibroplasts. One of us (D. T. S.) thanks his wife, Susan, for staining the reticulocytes. This investigation was supported in part by the National Science Foundation grant no. GB31763X and by the U.S. Public Health Service grant no. GM06966. One of us (D. T. S.) was supported by the National Institutes of Health Training grant no. 5-T1-GM-86.
Funders:
Funding AgencyGrant Number
NSFGB 31763X
NIHGM06966
NIH Predoctoral Fellowship5-T1-GM-86
Issue or Number:2
Record Number:CaltechAUTHORS:20160613-145751806
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160613-145751806
Official Citation:Daniel T. Simmons, James H. Strauss, Translation of Sindbis virus 26 S RNA and 49 S RNA in lysates of rabbit reticulocytes, Journal of Molecular Biology, Volume 86, Issue 2, 1974, Pages 397-409, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(74)90027-8. (http://www.sciencedirect.com/science/article/pii/0022283674900278)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67887
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:13 Jun 2016 22:46
Last Modified:03 Oct 2019 10:09

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