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Replication of Sindbis Virus I. Relative Size and Genetic Content of 26 s and 49 s RNA

Simmons, Daniel T. and Strauss, James H. (1972) Replication of Sindbis Virus I. Relative Size and Genetic Content of 26 s and 49 s RNA. Journal of Molecular Biology, 71 (3). pp. 599-613. ISSN 0022-2836. http://resolver.caltech.edu/CaltechAUTHORS:20160613-145752389

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Abstract

The genome of Sindbis virus, 49 s RNA, is a single, intact polynucleotide chain having a molecular weight of 4.3±0.3 x 10^6 daltons. This has been determined using a variety of methods including polyacrylamide gel electrophoresis, sedimentation after reaction with formaldehyde, determination of the molecular weights of the double-stranded forms of Sindbis-specific RNA and hybridization competition. The second major species of single-stranded RNA made after infection with Sindbis, 26 s RNA, has been found to have a molecular weight of 1.6 x 10^8 daltons as determined by sedimentation in dimethylsulfoxide. Hybridization-competition experiments carried out between these two species of RNA, using double-stranded forms of Sindbis RNA isolated from infected cells, showed that 26 s RNA contains only one-third of the base sequences in 49 s RNA and thus represents a unique fraction of the viral genome.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0022-2836(72)80026-3DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283672800263PublisherArticle
Additional Information:© 1972 Elsevier Ltd. Received 14 February 1972, and in revised form 10 July 1972. We are pleased to acknowledge the expert technical assistance of Edith M. Lenches and Mildred Quisenberry Hoover. We thank Dr. Ellen G. Strauss and John S. Pierce for their helpful editorial advice for the construction of this manuscript. This investigation was supported in part by the National Science Foundation grant no. GB13508 and by the U.S. Public Health Service grant no. GM06965. One of us (D. T. S.) was supported by the National Institutes of Health training grant no. 5-Tl-GM86.
Funders:
Funding AgencyGrant Number
NSFGB13508
NIHGM06965
NIH Predoctoral Fellowship5-T1-GM86
Record Number:CaltechAUTHORS:20160613-145752389
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160613-145752389
Official Citation:Daniel T. Simmons, James H. Strauss, Replication of Sindbis virus, Journal of Molecular Biology, Volume 71, Issue 3, 1972, Pages 599-613, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(72)80026-3. (http://www.sciencedirect.com/science/article/pii/S0022283672800263)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67889
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:13 Jun 2016 23:31
Last Modified:13 Jun 2016 23:32

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