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The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter

Borths, Elizabeth L. and Locher, Kaspar P. and Lee, Allen T. and Rees, Douglas C. (2002) The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter. Proceedings of the National Academy of Sciences of the United States of America, 99 (26). pp. 16642-16647. ISSN 0027-8424. PMCID PMC139197. https://resolver.caltech.edu/CaltechAUTHORS:BORpnas02

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Abstract

Bacterial binding protein-dependent ATP binding cassette (ABC) transporters facilitate uptake of essential nutrients. The crystal structure of Escherichia coli BtuF, the protein that binds vitamin B12 and delivers it to the periplasmic surface of the ABC transporter BtuCD, reveals a bi-lobed fold resembling that of the ferrichrome binding protein FhuD. B12 is bound in the "base-on" conformation in a deep cleft formed at the interface between the two lobes of BtuF. A stable complex between BtuF and BtuCD (with the stoichiometry BtuC2D2F) is demonstrated to form in vitro and was modeled using the individual crystal structures. Two surface glutamates from BtuF may interact with arginine residues on the periplasmic surface of the BtuCD transporter. These glutamate and arginine residues are conserved among binding proteins and ABC transporters mediating iron and B12 uptake, suggesting that they may have a role in docking and the transmission of conformational changes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.262659699DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc139197/PubMed CentralArticle
ORCID:
AuthorORCID
Rees, Douglas C.0000-0003-4073-1185
Additional Information:© 2002 by The National Academy of Sciences. Contributed by Douglas C. Rees and approved October 30, 2002. Published online before print December 10, 2002, 10.1073/pnas.262659699 Data deposition: The atomic coordinates have been deposited in the Protein Data Bank, www.rcsb.org (PDB ID code 1N2Z).
Issue or Number:26
PubMed Central ID:PMC139197
Record Number:CaltechAUTHORS:BORpnas02
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:BORpnas02
Official Citation:Elizabeth L. Borths, Kaspar P. Locher, Allen T. Lee, and Douglas C. Rees The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter PNAS 2002 99 (26) 16642-16647; published ahead of print December 10, 2002, doi:10.1073/pnas.262659699
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:679
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:14 Sep 2005
Last Modified:03 Jun 2020 23:00

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