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Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging

Zhang, Hao F. and Maslov, Konstantin and Stoica, George and Wang, Lihong V. (2006) Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging. Nature Biotechnology, 24 (7). pp. 848-851. ISSN 1087-0156. https://resolver.caltech.edu/CaltechAUTHORS:20160614-104743666

[img] PDF (Supplementary Fig. 1 An illustrative example showing the penetration depth of fPAM in tissue) - Supplemental Material
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[img] PDF (Supplementary Fig. 2 Images of vasculature in a rat acquired in vivo by fPAM at the isosbestic optical wavelength of 584 nm before, two days post, and five days post subcutaneous inoculation of BR_7C_5 tumor cells) - Supplemental Material
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[img] PDF (Supplementary Table 1 Comparison among the modern high-resolution microscopic imaging techniques, whose depth-to-resolution ratios are all greater than 100) - Supplemental Material
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[img] Video (AVI) (Supplementary Video 1. Movie for the composite volumetric visualization of a melanoma in the skin acquired in vivo) - Supplemental Material
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Abstract

Although optical absorption is strongly associated with the physiological status of biological tissue, existing high-resolution optical imaging modalities, including confocal microscopy, two-photon microscopy and optical coherence tomography, do not sense optical absorption directly. Furthermore, optical scattering prevents these methods from imaging deeper than ~1 mm below the tissue surface. Here we report functional photoacoustic microscopy (fPAM), which provides multiwavelength imaging of optical absorption and permits high spatial resolution beyond this depth limit with a ratio of maximum imaging depth to depth resolution greater than 100. Reflection mode, rather than orthogonal or transmission mode, is adopted because it is applicable to more anatomical sites than the others. fPAM is demonstrated with in vivo imaging of angiogenesis, melanoma, hemoglobin oxygen saturation (sO_2) of single vessels in animals and total hemoglobin concentration in humans.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/nbt1220DOIArticle
http://www.nature.com/nbt/journal/v24/n7/suppinfo/nbt1220_S1.htmlPublisherSupplementary Information
https://rdcu.be/b3zscPubMed CentralArticle
ORCID:
AuthorORCID
Maslov, Konstantin0000-0003-3408-8840
Stoica, George0000-0001-8071-4828
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2006 Macmillan Publishers Limited. Received 31 January; accepted 3 May; published online 25 June 2006. We thank O. Craciun, J. Oh, G. Ku, M.L. Li and G. Lungu for experimental assistance. This work was sponsored by National Institutes of Health grants R01 EB000712 and R01 NS46214. The authors declare that they have no competing financial interests.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 NS46214
Issue or Number:7
Record Number:CaltechAUTHORS:20160614-104743666
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160614-104743666
Official Citation:Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging Hao F Zhang, Konstantin Maslov, George Stoica & Lihong V Wang Nature Biotechnology 24, 848 - 851 (2006) Published online: 25 June 2006 | doi:10.1038/nbt1220
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67910
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Jun 2016 18:52
Last Modified:14 Apr 2020 22:15

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