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New method for isolation and sequence determination of 5'-terminal regions of bacteriophage φX174 in vitro mRNAs

Grohmann, Karel and Smith, Lloyd H. and Sinsheimer, Robert L. (1975) New method for isolation and sequence determination of 5'-terminal regions of bacteriophage φX174 in vitro mRNAs. Biochemistry, 14 (9). pp. 1951-1955. ISSN 0006-2960. http://resolver.caltech.edu/CaltechAUTHORS:20160614-165739565

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Abstract

We have determined the nucleotide sequences of the 5’-terminal oligonucleotides, produced by RNase T_1 digestion of bacteriophage φX174 mRNAs synthesized in vitro. The major sequences are: pppCpGp(Ap), pppApUpCpGp(Cp), pppAp(Ap)_2UpCp(Up)_2Gp(Gp), and pppAp(Ap)_3UpCp(Up)_2Gp(Gp). The sequences of several minor 5’-terminal oligonucleotides were also determined. For this research we have devised a simple isolation procedure, for the 5’-terminal oligonucleotides, based upon hydroxylapatite chromatography and two-dimensional thin-layer separation. This method allows for the rapid and quantitative recovery of all oligonucleotides containing 5’-triphosphate end groups and should be generally useful for sequence on 5’ termini of mRNAs.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bi00680a023DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi00680a023PublisherArticle
Additional Information:© 1975 American Chemical Society. Received November 25, 1974. This is a report of work supported in part by U.S. Public Health Service Grant No. GM 13554 and in part by US. Public Health Service Postdoctoral Research Fellowship No. GM 55249 to K.G.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM 13554
NIH Postdoctoral FellowshipGM 55249
Record Number:CaltechAUTHORS:20160614-165739565
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160614-165739565
Official Citation:New method for isolation and sequence determination of 5'-terminal regions of bacteriophage φX174 in vitro mRNAs Karel Grohmann, Lloyd H. Smith, and Robert L. Sinsheimer Biochemistry 1975 14 (9), 1951-1955 DOI: 10.1021/bi00680a023
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67930
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:15 Jun 2016 16:01
Last Modified:13 Feb 2019 18:06

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