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High-speed label-free functional photoacoustic microscopy of mouse brain in action

Yao, Junjie and Wang, Lidai and Yang, Joon-Mo and Maslov, Konstantin I. and Wong, Terence T. W. and Li, Lei and Huang, Chih-Hsien and Zou, Jun and Wang, Lihong V. (2015) High-speed label-free functional photoacoustic microscopy of mouse brain in action. Nature Methods, 12 (5). pp. 407-410. ISSN 1548-7091. PMCID PMC4428901.

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[img] PDF (Supplementary Figures 1–17, Supplementary Table 1 and Supplementary Notes 1–3) - Supplemental Material
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[img] Video (QuickTime) (Video 1: Three-dimensional rendering of mouse brain) - Supplemental Material
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[img] Video (QuickTime) (Video 2: Cross-sectional rendering of the mouse brain vasculature) - Supplemental Material
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[img] Video (QuickTime) (Video 3: PAM of cerebral responses to electrical stimulations to the hindlimbs) - Supplemental Material
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[img] Video (QuickTime) (Video 4: Volumetric rendering of oxygen saturation (sO_2)) - Supplemental Material
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[img] Video (QuickTime) (Video 5: PAM of cerebral sO_2 responses to electrical stimulations to the left hindlimb) - Supplemental Material
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We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle ReadCube access
Maslov, Konstantin I.0000-0003-3408-8840
Wong, Terence T. W.0000-0001-6399-758X
Li, Lei0000-0001-6164-2646
Zou, Jun0000-0002-9543-6135
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2015 Macmillan Publishers Limited. Received 25 April 2014; Accepted 20 February 2015; Published online 30 March 2015. The authors appreciate J. Ballard’s close reading of the manuscript. We thank C. Li, C. Chen, W. Chapman and J. Lee for discussions and technical support. This work was sponsored by US National Institutes of Health (NIH) grants DP1 EB016986 (NIH Director’s Pioneer Award), R01 CA186567 (NIH Director’s Transformative Research Award), 1S10 RR028864 and R01 CA159959 (to L.V.W.), and US National Science Foundation (NSF) grant CMMI-1131758 (to J.Z.). The bright-field microscopy was performed at the Alafi Neuroimaging Laboratory of the Hope Center for Neurological Disorders, Washington University School of Medicine, which is supported by the NIH Neuroscience Blueprint Center Core grant P30 NS057105. Author Contributions: J.Y., J.Z. and L.V.W. conceived of and designed the study. J.Y., J.-M.Y., K.I.M., L.W. and C.-H.H. constructed the imaging system. J.Y., T.T.W.W. and L.L. performed the experiments and analyzed the data. J.Y., L.W. and L.V.W. wrote the manuscript. L.V.W. supervised the whole study. All authors discussed the conceptual and practical implications of the methods. Competing Financial Interests: L.V.W. has a financial interest in Endra, Inc. and Microphotoacoustics, Inc., which, however, did not support this work. K.I.M. also has a financial interest in Microphotoacoustics, Inc.
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIH1S10 RR028864
NIHR01 CA159959
NIHP30 NS057105
Issue or Number:5
PubMed Central ID:PMC4428901
Record Number:CaltechAUTHORS:20160615-093234473
Persistent URL:
Official Citation:High-speed label-free functional photoacoustic microscopy of mouse brain in action Junjie Yao, Lidai Wang, Joon-Mo Yang, Konstantin I Maslov, Terence T W Wong, Lei Li, Chih-Hsien Huang, Jun Zou & Lihong V Wang Nature Methods 12, 407–410 (2015) doi:10.1038/nmeth.3336
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67935
Deposited By: Tony Diaz
Deposited On:15 Jun 2016 16:56
Last Modified:09 Mar 2020 13:19

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