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Purification and Properties of Bacteriophage MS2 and of its Ribonucleic Acid

Strauss, James H., Jr. and Sinsheimer, Robert L. (1963) Purification and Properties of Bacteriophage MS2 and of its Ribonucleic Acid. Journal of Molecular Biology, 7 (1). pp. 43-54. ISSN 0022-2836. doi:10.1016/S0022-2836(63)80017-0.

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Methods are described for the isolation in pure form of bacteriophage MS2 and of its RNA. The virus has a particle weight of 3·6 × 10^6, and from phosphorus content is estimated to be 32% RNA by weight. The particle weight of the isolated RNA is 1·05 ± 0·1 × 10^6, indicating that there is one molecule of RNA per virus particle. The RNA is shown to have several unusual properties. In 0·2 M-NaCl it has a very small radius of gyration and a large sedimentation coefficient for its molecular weight, indicating a very compact structure. Upon heating the RNA in 0·14 M-NaCl solutions, the ultraviolet absorbancy transition has a midpoint at 76°C, suggesting a high degree of hydrogen bonding. In lower salt concentrations the RNA aggregates; in 0·02 M-NaCl the observed molecular weight is twice that in 0·2 M-NaCl, and in 0·003 M-tris it is 3 times this value.

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Additional Information:© 1963 Elsevier Ltd. Received 18 February 1963, Revised 12 April 1963. The determination of the base composition and of the extinction coefficient of MS2 RNA were performed by Mr. John Sedat. This research was supported in part by research grant RG6965 and training grant 2G-86 from the U.S. Public Health Service.
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U.S. Public Health Service (USPHS)RG6965
NIH Predoctoral Fellowship2G86
Issue or Number:1
Record Number:CaltechAUTHORS:20160616-110130048
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Official Citation:James H. Strauss, Robert L. Sinsheimer, Purification and properties of bacteriophage MS2 and of its ribonucleic acid, Journal of Molecular Biology, Volume 7, Issue 1, 1963, Pages 43-54, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67972
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 19:12
Last Modified:11 Nov 2021 03:57

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