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Purification and Properties of Bacteriophage MS2 and of its Ribonucleic Acid

Strauss, James H., Jr. and Sinsheimer, Robert L. (1963) Purification and Properties of Bacteriophage MS2 and of its Ribonucleic Acid. Journal of Molecular Biology, 7 (1). pp. 43-54. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160616-110130048

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Abstract

Methods are described for the isolation in pure form of bacteriophage MS2 and of its RNA. The virus has a particle weight of 3·6 × 10^6, and from phosphorus content is estimated to be 32% RNA by weight. The particle weight of the isolated RNA is 1·05 ± 0·1 × 10^6, indicating that there is one molecule of RNA per virus particle. The RNA is shown to have several unusual properties. In 0·2 M-NaCl it has a very small radius of gyration and a large sedimentation coefficient for its molecular weight, indicating a very compact structure. Upon heating the RNA in 0·14 M-NaCl solutions, the ultraviolet absorbancy transition has a midpoint at 76°C, suggesting a high degree of hydrogen bonding. In lower salt concentrations the RNA aggregates; in 0·02 M-NaCl the observed molecular weight is twice that in 0·2 M-NaCl, and in 0·003 M-tris it is 3 times this value.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0022-2836(63)80017-0DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283663800170PublisherArticle
Additional Information:© 1963 Elsevier Ltd. Received 18 February 1963, Revised 12 April 1963. The determination of the base composition and of the extinction coefficient of MS2 RNA were performed by Mr. John Sedat. This research was supported in part by research grant RG6965 and training grant 2G-86 from the U.S. Public Health Service.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
NIH Predoctoral Fellowship2G86
Issue or Number:1
Record Number:CaltechAUTHORS:20160616-110130048
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160616-110130048
Official Citation:James H. Strauss, Robert L. Sinsheimer, Purification and properties of bacteriophage MS2 and of its ribonucleic acid, Journal of Molecular Biology, Volume 7, Issue 1, 1963, Pages 43-54, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(63)80017-0. (http://www.sciencedirect.com/science/article/pii/S0022283663800170)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67972
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 19:12
Last Modified:03 Oct 2019 10:10

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