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The structure of the DNA of bacteriophage φX174: IV. Pyrimidine Sequences

Hall, John B. and Sinsheimer, Robert L. (1963) The structure of the DNA of bacteriophage φX174: IV. Pyrimidine Sequences. Journal of Molecular Biology, 6 (2). pp. 115-127. ISSN 0022-2836. doi:10.1016/S0022-2836(63)80127-8.

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Apurinic acid was prepared from ^(14)C-labeled DNA of the bacteriophage φX174 with unlabeled calf thymus DNA as carrier. The terminal phosphates of the pyrimidine tracts were removed by treatment with the alkaline phosphatase of E. coli and the resulting mixture fractionated on a column of DEAE-Sephadex into fragments of uniform chain length (isopliths) up to undecanucleotides.

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Additional Information:© 1963 Elsevier Ltd. Received 26 September 1962. We wish to thank Dr. I. R. Lehman for his generous gift of E. coli phosphodiesterase. We would also like to express our appreciation to Miss Kathleen Gaines and Mr. John Sedat for their excellent assistance. This research was supported, in part, by U.S. Public Health Service Grants RG6965 and H3103.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
U.S. Public Health Service (USPHS)H3103
Issue or Number:2
Record Number:CaltechAUTHORS:20160616-133842222
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Official Citation:John B. Hall, Robert L. Sinsheimer, The structure of the DNA of bacteriophage φX174, Journal of Molecular Biology, Volume 6, Issue 2, 1963, Pages 115-IN3, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67978
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 19:04
Last Modified:11 Nov 2021 03:57

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