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The Replication of Bacteriophage MS2: 1. Transfer of Parental Nucleic Acid to Progeny Phage

Davis, James E. and Sinsheimer, Robert L. (1963) The Replication of Bacteriophage MS2: 1. Transfer of Parental Nucleic Acid to Progeny Phage. Journal of Molecular Biology, 6 (3). pp. 203-207. ISSN 0022-2836. doi:10.1016/S0022-2836(63)80069-8. https://resolver.caltech.edu/CaltechAUTHORS:20160616-133842497

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Abstract

Conditions and procedures are described for the growth and assay of bacteriophage MS2, an RNA-containing phage. Procedures are described for the preparation of ^(15)N^(32)P-labeled MS2 phage, and for the use of such labeled phage in the measurement of transfer of parental RNA to progeny phage. No evidence for any type of conservative or semi-conservative transfer is found and dispersive transfer is limited to 3%.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0022-2836(63)80069-8DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283663800698PublisherArticle
Additional Information:© 1963 Elsevier Ltd. Received 20 November 1962. The able technical assistance of Mrs. Shizuko Yagi is gratefully acknowledged. One of us (J.E.D.) is a Postdoctoral fellow of the U.S. Public Health Service. This research was supported in part by a grant from the U.S. Public Health Service No. RG6965.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
Issue or Number:3
DOI:10.1016/S0022-2836(63)80069-8
Record Number:CaltechAUTHORS:20160616-133842497
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160616-133842497
Official Citation:James E. Davis, Robert L. Sinsheimer, The replication of bacteriophage MS2, Journal of Molecular Biology, Volume 6, Issue 3, 1963, Pages 203-207, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(63)80069-8. (http://www.sciencedirect.com/science/article/pii/S0022283663800698)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67979
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 19:17
Last Modified:11 Nov 2021 03:57

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