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Inhibition of Protein Synthesis in E. coli Protoplasts by Actinomycin-D

Haywood, Anne M. and Sinsheimer, Robert L. (1963) Inhibition of Protein Synthesis in E. coli Protoplasts by Actinomycin-D. Journal of Molecular Biology, 6 (3). pp. 247-249. ISSN 0022-2836. doi:10.1016/S0022-2836(63)80074-1.

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Actinomycin-D has been reported to inhibit DNA-dependent RNA synthesis (Goldberg & Rabinowitz, 1962; Hurwitz, Furth, Malamy & Alexander, 1962), but to allow RNA viruses to continue to replicate in animal cells (Reich, Franklin, Shatkin & Tatum, 1961, 1962.) In Gram-positive bacteria this inhibition of RNA synthesis has been followed by an inhibition of protein synthesis (Kirk, 1960; Levinthal, Keynan & Higa, 1962). Actinomycin-D has been reported not to affect E. coli (Hurwitz et al., 1962), most likely because the cell wall is impermeable to actinomycin. We have observed that actinomycin-D at 10 y/ml. will strongly inhibit protein synthesis in protoplasts of E.coli C-3000 without inhibiting the growth of an RNA bacteriophage, MS2 (Davis, Strauss & Sinsheimer, 1961).

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Additional Information:© 1963 Elsevier Ltd. Received 14 January 1963. This research was supported in part by grant RG 6965 from the National Institutes of Health.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
Issue or Number:3
Record Number:CaltechAUTHORS:20160616-133842732
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Official Citation:Anne M. Haywood, Robert L. Sinsheimer, Inhibition of protein synthesis in E. coli protoplasts by actinomycin-D, Journal of Molecular Biology, Volume 6, Issue 3, 1963, Pages 247-249, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67980
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 19:15
Last Modified:11 Nov 2021 03:58

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