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Inhibition of Protein Synthesis in E. coli Protoplasts by Actinomycin-D

Haywood, Anne M. and Sinsheimer, Robert L. (1963) Inhibition of Protein Synthesis in E. coli Protoplasts by Actinomycin-D. Journal of Molecular Biology, 6 (3). pp. 247-249. ISSN 0022-2836. doi:10.1016/S0022-2836(63)80074-1. https://resolver.caltech.edu/CaltechAUTHORS:20160616-133842732

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Abstract

Actinomycin-D has been reported to inhibit DNA-dependent RNA synthesis (Goldberg & Rabinowitz, 1962; Hurwitz, Furth, Malamy & Alexander, 1962), but to allow RNA viruses to continue to replicate in animal cells (Reich, Franklin, Shatkin & Tatum, 1961, 1962.) In Gram-positive bacteria this inhibition of RNA synthesis has been followed by an inhibition of protein synthesis (Kirk, 1960; Levinthal, Keynan & Higa, 1962). Actinomycin-D has been reported not to affect E. coli (Hurwitz et al., 1962), most likely because the cell wall is impermeable to actinomycin. We have observed that actinomycin-D at 10 y/ml. will strongly inhibit protein synthesis in protoplasts of E.coli C-3000 without inhibiting the growth of an RNA bacteriophage, MS2 (Davis, Strauss & Sinsheimer, 1961).


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0022-2836(63)80074-1DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283663800741PublisherArticle
Additional Information:© 1963 Elsevier Ltd. Received 14 January 1963. This research was supported in part by grant RG 6965 from the National Institutes of Health.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
Issue or Number:3
DOI:10.1016/S0022-2836(63)80074-1
Record Number:CaltechAUTHORS:20160616-133842732
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160616-133842732
Official Citation:Anne M. Haywood, Robert L. Sinsheimer, Inhibition of protein synthesis in E. coli protoplasts by actinomycin-D, Journal of Molecular Biology, Volume 6, Issue 3, 1963, Pages 247-249, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(63)80074-1. (http://www.sciencedirect.com/science/article/pii/S0022283663800741)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67980
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 19:15
Last Modified:11 Nov 2021 03:58

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