Sedat, John and Sinsheimer, Robert L. (1964) Structure of the DNA of Bacteriophage ΦX174: V. Purine Sequences. Journal of Molecular Biology, 9 (2). pp. 489-497. ISSN 0022-2836. doi:10.1016/S0022-2836(64)80221-7. https://resolver.caltech.edu/CaltechAUTHORS:20160617-071022953
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Abstract
Methods are described for the preparation of DNA, specifically labeled with carbon-14 in the purines, from bacteriophage ΦX174. This DNA, mixed with unlabeled calf thymus DNA as a carrier, has been degraded to purine tracts by hydrazinolysis and subsequent alkaline hydrolysis. The purine tracts have been separated according to magnitude by chromatography on a column of DEAE-Sephadex, at 55°C in the presence of 7 M-urea.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1964 Elsevier Ltd. . , Available online 15 May 2009. We acknowledge the assistance of Mrs. Regine Weil in the growth and purification of the purine-labeled ΦX and of Mrs. Carolyn Nagler in the chromatographic refractlonations, This research was supported, in part, by a grant, RG6965, from the U.S. Public Health Service. | |||||||||
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| Issue or Number: | 2 | |||||||||
| DOI: | 10.1016/S0022-2836(64)80221-7 | |||||||||
| Record Number: | CaltechAUTHORS:20160617-071022953 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160617-071022953 | |||||||||
| Official Citation: | John Sedat, Robert L. Sinsheimer, Structure of the DNA of bacteriophage ΦX174, Journal of Molecular Biology, Volume 9, Issue 2, 1964, Pages 489-497, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(64)80221-7. (http://www.sciencedirect.com/science/article/pii/S0022283664802217) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 68455 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 17 Jun 2016 19:10 | |||||||||
| Last Modified: | 11 Nov 2021 04:00 |
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