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Structure of the DNA of Bacteriophage ΦX174: V. Purine Sequences

Sedat, John and Sinsheimer, Robert L. (1964) Structure of the DNA of Bacteriophage ΦX174: V. Purine Sequences. Journal of Molecular Biology, 9 (2). pp. 489-497. ISSN 0022-2836. doi:10.1016/S0022-2836(64)80221-7.

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Methods are described for the preparation of DNA, specifically labeled with carbon-14 in the purines, from bacteriophage ΦX174. This DNA, mixed with unlabeled calf thymus DNA as a carrier, has been degraded to purine tracts by hydrazinolysis and subsequent alkaline hydrolysis. The purine tracts have been separated according to magnitude by chromatography on a column of DEAE-Sephadex, at 55°C in the presence of 7 M-urea.

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Additional Information:© 1964 Elsevier Ltd. . , Available online 15 May 2009. We acknowledge the assistance of Mrs. Regine Weil in the growth and purification of the purine-labeled ΦX and of Mrs. Carolyn Nagler in the chromatographic refractlonations, This research was supported, in part, by a grant, RG6965, from the U.S. Public Health Service.
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U.S. Public Health Service (USPHS)RG6965
Issue or Number:2
Record Number:CaltechAUTHORS:20160617-071022953
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Official Citation:John Sedat, Robert L. Sinsheimer, Structure of the DNA of bacteriophage ΦX174, Journal of Molecular Biology, Volume 9, Issue 2, 1964, Pages 489-497, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68455
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 19:10
Last Modified:11 Nov 2021 04:00

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