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Structure of the DNA of Bacteriophage ΦX174: V. Purine Sequences

Sedat, John and Sinsheimer, Robert L. (1964) Structure of the DNA of Bacteriophage ΦX174: V. Purine Sequences. Journal of Molecular Biology, 9 (2). pp. 489-497. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160617-071022953

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Abstract

Methods are described for the preparation of DNA, specifically labeled with carbon-14 in the purines, from bacteriophage ΦX174. This DNA, mixed with unlabeled calf thymus DNA as a carrier, has been degraded to purine tracts by hydrazinolysis and subsequent alkaline hydrolysis. The purine tracts have been separated according to magnitude by chromatography on a column of DEAE-Sephadex, at 55°C in the presence of 7 M-urea.


Item Type:Article
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http://dx.doi.org/10.1016/S0022-2836(64)80221-7DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283664802217PublisherArticle
Additional Information:© 1964 Elsevier Ltd. . , Available online 15 May 2009. We acknowledge the assistance of Mrs. Regine Weil in the growth and purification of the purine-labeled ΦX and of Mrs. Carolyn Nagler in the chromatographic refractlonations, This research was supported, in part, by a grant, RG6965, from the U.S. Public Health Service.
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Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
Issue or Number:2
Record Number:CaltechAUTHORS:20160617-071022953
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160617-071022953
Official Citation:John Sedat, Robert L. Sinsheimer, Structure of the DNA of bacteriophage ΦX174, Journal of Molecular Biology, Volume 9, Issue 2, 1964, Pages 489-497, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(64)80221-7. (http://www.sciencedirect.com/science/article/pii/S0022283664802217)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68455
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 19:10
Last Modified:03 Oct 2019 10:14

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