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In Vitro Synthesis and Properties of a φX DNA-RNA Hybrid

Sinsheimer, Robert L. and Lawrence, Marianne (1964) In Vitro Synthesis and Properties of a φX DNA-RNA Hybrid. Journal of Molecular Biology, 8 (2). pp. 289-296. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160617-074730405

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Abstract

When the single-stranded DNA of bacteriophage φX174 is the primer for E. coli RNA polymerase, a DNA-RNA hybrid is formed. This hybrid, which has been isolated from a CsCl density gradient, melts over a relatively narrow temperature range. The hybrid is infective to bacterial protoplasts and the u.v. sensitivity of this infectivity is distinct from that of the free DNA. If the hybrid is denatured with formamide, the RNA may be recovered as fragments with a broad range of sedimentation coefficient from 6 to 14 s.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0022-2836(64)80138-8DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283664801388PublisherArticle
Additional Information:© 1964 Elsevier Ltd. Received 10 October 1963. We are much indebted to Dr. T. August and Dr. M. Chamberlin for assistance in the development of procedures for isolation of the RNA polymerase. dAT was a generous gift from Dr. R. Baldwin. This research was supported in part by a grant no, RG6965 from the U.S. Public Health Service.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
Issue or Number:2
Record Number:CaltechAUTHORS:20160617-074730405
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160617-074730405
Official Citation:Robert L. Sinsheimer, Marianne Lawrence, In Vitro synthesis and properties of a φX DNA-RNA hybrid, Journal of Molecular Biology, Volume 8, Issue 2, 1964, Pages 289-296, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(64)80138-8. (http://www.sciencedirect.com/science/article/pii/S0022283664801388)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68459
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 18:48
Last Modified:03 Oct 2019 10:14

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