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The Process of Infection with Bacteriophage φX174: V. Inactivation of the Phage-Bacterium Complex by Decay of ^(32)P incorporated in the Infecting Particle

Denhardt, David T. and Sinsheimer, Robert L. (1965) The Process of Infection with Bacteriophage φX174: V. Inactivation of the Phage-Bacterium Complex by Decay of ^(32)P incorporated in the Infecting Particle. Journal of Molecular Biology, 12 (3). pp. 663-673. ISSN 0022-2836. http://resolver.caltech.edu/CaltechAUTHORS:20160617-090436276

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Abstract

φX bacteriophage containing 2 to 4 ^(32)P atoms per particle were used to infect cells in non-radioactive medium. The phage and early complexes were killed by ^(32)P-decay with an efficiency of 1; mature complexes were inactivated with an efficiency of about 0·2, even though unlabeled, infective replicative form φX DNA had been synthesized in them. At about the same time as progeny single-strand DNA was synthesized, the complexes became completely refractory to inactivation by ^(32)P decay. In chloramphenicol the efficiency of ^(32)P decay inactivation in the complexes also decreased to about 0·2; thereafter complexes formed, in chloramphenicol, from cells that had not been starved became fully resistant to inactivation, whereas complexes formed from starved cells did not.


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http://dx.doi.org/10.1016/S0022-2836(65)80320-5DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283665803205PublisherArticle
Additional Information:© 1965 Elsevier Ltd. Received 29 December 1964, and in revised form 22 March 1965. This work was reported in a thesis submitted to the California Institute of Technology by one of us (D.T.D.) in partial fulfillment of the requirements for the degree of Doctor of Philosophy. Financial support was provided by fellowships from the National Science Foundation and the U.S. Public Health Service and by grant RG-6965 of the U.S. Public Health Service. Many stimulating discussions with Mr Michael Yarus are acknowledged with pleasure.
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U.S. Public Health Service (USPHS)RG 6569
Record Number:CaltechAUTHORS:20160617-090436276
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160617-090436276
Official Citation:David T. Denhardt, Robert L. Sinsheimer, The process of infection with bacteriophage φX174, Journal of Molecular Biology, Volume 12, Issue 3, 1965, Pages 663-673, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(65)80320-5. (http://www.sciencedirect.com/science/article/pii/S0022283665803205)
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ID Code:68467
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 18:16
Last Modified:17 Jun 2016 18:16

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