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The Process of Infection with Bacteriophage φX174: VI. Inactivation of Infected Complexes by Ultraviolet Irradiation

Denhardt, David T. and Sinsheimer, Robert L. (1965) The Process of Infection with Bacteriophage φX174: VI. Inactivation of Infected Complexes by Ultraviolet Irradiation. Journal of Molecular Biology, 12 (3). pp. 674-694. ISSN 0022-2836. http://resolver.caltech.edu/CaltechAUTHORS:20160617-091351317

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Abstract

The sensitivities to ultraviolet irradiation of complexes formed by infection with bacteriophage φX174 in Escherichia coli C, E. coli C_s (a strain lacking the capacity to perform host cell reactivation), and E. coli CR (a strain requiring thymine) were determined at various wavelengths, at various stages of development, and under various growth conditions. Neither irradiated bacteriophage φX174 nor irradiated free φX DNA is subject to host cell reactivation; however, irradiated replicative form φX DNA is. The target that is observed in the ultraviolet inactivation curves of the complex after three minutes of infection is also subject to host cell reactivation. In the absence of host cell reactivation, the ultraviolet inactivation curves suggest the existence of either one or a very few sensitive targets in the infected cell.


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http://dx.doi.org/10.1016/S0022-2836(65)80321-7DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283665803217PublisherArticle
Additional Information:© 1965 Elsevier Ltd. Received 29 December 1964, and in revised form 22 March, 1965. This work was supported by predoctoral fellowships from the National Science Foundation and the National Institutes of Health end by grant RG-6965 from t he U.S . Public Health Service; it was contained in a thesis submitted to the California Institute of Technology by one of u s (D.T.D.) in partial fulfillment of the requirement s for the degree of Doctor of Philosophy. The data in Fig. 1 were obtained in collaboration with Michael Yarns. We wish to acknowledge many useful and stimulating discussions with Mr Yarns, for out of these discussions many of the ideas expressed in this paper evolved. The action spectra dat a for φX complexes presented in Fig. 12 were determined with the competent assistance of Mrs Barbara Furman Attardi. The monochromator used in this work was generously made available by Dr Max Delbrück, Valuable discussions with Dr Delbrück helped clarify many of the points made in this paper.
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U.S. Public Health Service (USPHS)RG6965
Record Number:CaltechAUTHORS:20160617-091351317
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160617-091351317
Official Citation:David T. Denhardt, Robert L. Sinsheimer, The process of infection with bacteriophage φX174, Journal of Molecular Biology, Volume 12, Issue 3, 1965, Pages 674-694, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(65)80321-7. (http://www.sciencedirect.com/science/article/pii/S0022283665803217)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68469
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 17:44
Last Modified:17 Jun 2016 17:44

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