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In vivo imaging of subcutaneous structures using functional photoacoustic microscopy

Zhang, Hao F. and Maslov, Konstantin and Wang, Lihong V. (2007) In vivo imaging of subcutaneous structures using functional photoacoustic microscopy. Nature Protocols, 2 (4). pp. 797-804. ISSN 1754-2189. doi:10.1038/nprot.2007.108. https://resolver.caltech.edu/CaltechAUTHORS:20160617-094419130

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Abstract

Functional photoacoustic microscopy (fPAM) is a hybrid technology that permits noninvasive imaging of the optical absorption contrast in subcutaneous biological tissues. fPAM uses a focused ultrasonic transducer to detect high-frequency photoacoustic (PA) signals. Volumetric images of biological tissues can be formed by two-dimensional raster scanning, and functional parameters can be further extracted from spectral measurements. fPAM is safe and applicable to animals as well as humans. This protocol provides guidelines for parameter selection, system alignment, imaging operation, laser safety and data processing for in vivo fPAM. It currently takes ~100 min to carry out this protocol, including ~50 min for data acquisition using a 10-Hz pulse-repetition-rate laser system. The data acquisition time, however, can be significantly reduced by using a laser system with a higher pulse repetition rate.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/nprot.2007.108DOIArticle
https://rdcu.be/bpAIrPublisherFree ReadCube access
ORCID:
AuthorORCID
Maslov, Konstantin0000-0003-3408-8840
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2007 Macmillan Publishers Limited. Published online 5 April 2007. We thank G. Stoica, O. Craciun, J. Oh, G. Ku, M.-L. Li, X. Xie, G. Lungu, R. Zemp and M. Sivaramakrishnan for experimental assistance. This work was sponsored by grants from the National Institutes of Health (R01 EB000712 and R01 NS46214 to L.V.W.). The authors declare no competing financial interests.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 NS46214
Issue or Number:4
DOI:10.1038/nprot.2007.108
Record Number:CaltechAUTHORS:20160617-094419130
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160617-094419130
Official Citation:In vivo imaging of subcutaneous structures using functional photoacoustic microscopy Hao F Zhang, Konstantin Maslov & Lihong V Wang Nature Protocols 2, - 797 - 804 (2007) Published online: 5 April 2007 | doi:10.1038/nprot.2007.108
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68472
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Jun 2016 17:42
Last Modified:11 Nov 2021 04:00

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