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The Process of Infection with Bacteriophage ΦX174: X. Mutations in a ΦX Lysis Gene

Hutchison, Clyde A., III and Sinsheimer, Robert L. (1966) The Process of Infection with Bacteriophage ΦX174: X. Mutations in a ΦX Lysis Gene. Journal of Molecular Biology, 18 (3). 429-IN2. ISSN 0022-2836. doi:10.1016/S0022-2836(66)80035-9.

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The ability of conditional lethal mutants of phage ΦX174 to induce host cell lysis during infection under restrictive conditions has been studied. We have found amber (am) and temperature-sensitive (ts) mutants which present a variety of alterations in the normal lytic process. In particular, there is a class of am mutants which do not produce cell lysis but otherwise replicate normally in the restrictive host. These mutants constitute a single complementation group. The existence of these mutants implicates a phage-coded protein in the lytic process. This protein is not an essential structural component of the phage, since normal phage particles are produced in the absence of lysis.

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Additional Information:© 1966 Elsevier Ltd. Received 12 January 1966. We thank D. Pfeifer, S. G. Krane, M. Yarus, R. Levine, and G. Streisinger for permission to mention unpublished results. We also wish to acknowledge the capable technical assistance of Alma Shafer and Marianne Lawrence. This work was supported in part by grants RG6965 and GM13554 of the U.S. Public Health Service.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
U.S. Public Health Service (USPHS)GM13554
Issue or Number:3
Record Number:CaltechAUTHORS:20160617-102055428
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Official Citation:Clyde A. Hutchison, Robert L. Sinsheimer, The process of infection with bacteriophage ΦX174, Journal of Molecular Biology, Volume 18, Issue 3, 1966, Pages 429-IN2, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68476
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2016 17:29
Last Modified:11 Nov 2021 04:00

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