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Seeing Through the Surface: Non-invasive Characterization of Biomaterial-Tissue Interactions Using Photoacoustic Microscopy

Zhang, Yu Shrike and Wang, Lihong V. and Xia, Younan (2016) Seeing Through the Surface: Non-invasive Characterization of Biomaterial-Tissue Interactions Using Photoacoustic Microscopy. Annals of Biomedical Engineering, 44 (3). pp. 649-666. ISSN 0090-6964. PMCID PMC4792739. http://resolver.caltech.edu/CaltechAUTHORS:20160617-143506465

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Abstract

At the intersection of life sciences, materials science, engineering, and medicine, regenerative medicine stands out as a rapidly progressing field that aims at retaining, restoring, or augmenting tissue/organ functions to promote the human welfare. While the field has witnessed tremendous advancements over the past few decades, it still faces many challenges. For example, it has been difficult to visualize, monitor, and assess the functions of the engineered tissue/organ constructs, particularly when three-dimensional scaffolds are involved. Conventional approaches based on histology are invasive and therefore only convey end-point assays. The development of volumetric imaging techniques such as confocal and ultrasonic imaging has enabled direct observation of intact constructs without the need of sectioning. However, the capability of these techniques is often limited in terms of penetration depth and contrast. In comparison, the recently developed photoacoustic microscopy (PAM) has allowed us to address these issues by integrating optical and ultrasonic imaging to greatly reduce the effect of tissue scattering of photons with one-way ultrasound detection while retaining the high optical absorption contrast. PAM has been successfully applied to a number of studies, such as observation of cell distribution, monitoring of vascularization, and interrogation of biomaterial degradation. In this review article, we highlight recent progress in non-invasive and volumetric characterization of biomaterial–tissue interactions using PAM. We also discuss challenges ahead and envision future directions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/s10439-015-1485-2DOIArticle
https://rdcu.be/boIg5PublisherFree ReadCube access
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792739PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2015 Biomedical Engineering Society. Received 20 August 2015; accepted 8 October 2015; published online 15 October 2015. This work was supported in part by startup funds from the Georgia Institute of Technology and NIH Grants DP1 OD000798 (NIH Director’s Pioneer Award) and R01 AR060820. The authors would like to thank Dr. Yu Wang and Dr. Li Li for their assistance in OR-PAM–FCM and OR-PAM–OCT imaging of melanoma cell-scaffold interactions. Conflict of Interest: L. V. Wang has a financial interest in Endra, Inc., and Microphotoacoustics, Inc., which, however, did not support this work; all other authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
NIHDP1 OD000798
NIHR01 AR060820
Georgia Institute of TechnologyUNSPECIFIED
Subject Keywords:Biomedical imaging; Non-invasive; Photoacoustic microscopy; Regenerative medicine; Tissue engineering
PubMed Central ID:PMC4792739
Record Number:CaltechAUTHORS:20160617-143506465
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160617-143506465
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68492
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 Jun 2016 18:15
Last Modified:27 Feb 2019 18:58

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