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Process of Infection with Bacteriophage øX174: XXIII. DNA Synthesis in Cells infected by a Coat Protein Mutant

Knippers, Rolf and Sinsheimer, Robert L. (1968) Process of Infection with Bacteriophage øX174: XXIII. DNA Synthesis in Cells infected by a Coat Protein Mutant. Journal of Molecular Biology, 35 (3). pp. 591-605. ISSN 0022-2836. doi:10.1016/S0022-2836(68)80016-6.

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Single-stranded progeny DNA is not formed when host cells are infected at the restrictive temperature with a temperature-sensitive coat protein mutant of øX174. Once begun, the synthesis of single-stranded DNA is interrupted when an infected culture is shifted from the permissive to the restrictive temperature. The synthesis of double-stranded “replicative form” DNA is observed under restrictive conditions. Upon shifting an infected culture from restrictive to permissive conditions after 30 minutes of infection, single-stranded DNA synthesis starts almost immediately. In this instance, the initial single-stranded viral DNA is formed out of the pre-formed double-stranded replicative form DNA by degradation of the complementary strand. The attachment of coat protein to the viral strand and the release of the viral strand from the double-stranded precursor appear to take place simultaneously.

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Additional Information:© 1968 Elsevier Ltd. Received 15 January 1968, and in revised form 5 June 1968. This research was supported in part by grant GM 13554 from the U.S. Public Health Service.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM 13554
Issue or Number:3
Record Number:CaltechAUTHORS:20160620-065435192
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Official Citation:Rolf Knippers, Robert L. Sinsheimer, Process of infection with bacteriophage øX174, Journal of Molecular Biology, Volume 35, Issue 3, 1968, Pages 591-605, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68499
Deposited By: Ruth Sustaita
Deposited On:20 Jun 2016 19:22
Last Modified:11 Nov 2021 04:00

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