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The Replication of Bacteriophage MS2: VI. Interaction between Bacteriophage RNA and Cellular Components in MS2-infected Escherichia coli

Godson, Godfrey N. and Sinsheimer, Robert L. (1967) The Replication of Bacteriophage MS2: VI. Interaction between Bacteriophage RNA and Cellular Components in MS2-infected Escherichia coli. Journal of Molecular Biology, 23 (3). pp. 495-521. ISSN 0022-2836. http://resolver.caltech.edu/CaltechAUTHORS:20160620-070308711

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Abstract

Almost immediately after infection of Escherichia coli cells with [^(32)P]MS2, parental RNA appears in cell lysates associated with the 30 s ribosome subunit and with polysomes; a small amount is found as unbound, free RNA. At early times after infection (zero to four minutes) no parental RNA is associated with the 70 s monosomes or with the 50 s ribosome subunits. After four to five minutes of infection, the distribution of these components is such that approximately 30% of the total [^(32)P]parental RNA in the cells is associated with the polysomes, 38% is associated with the 30 s ribosome subunit, 15% is free RNA and 16% is residual whole phage. At later times (four to ten minutes), coincident with the time of increase in ribonuclease-resistant parental RNA, a new component containing [^(32)P]parental RNA appears, sedimenting just in front of the 50 s ribosome subunit. The ribonuclease-resistant 12 s RNA (“core” RNA, Fenwick, Erikson & Franklin, 1964) is present in both this 50 s region and in the polysomes: none is present in the 30 s or free RNA regions. The ribonuclease-resistant 12 s RNA probably appears first in the 50 s region and later in the polysome region. The [^(32)P]parental RNA of cells infected for eight minutes can be dissociated, in low Mg^(2+) concentration, from the polysomes (and can also be derived from the 50 s component) as a fast sedimenting component of 40 s.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0022-2836(67)80121-9DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283667801219PublisherArticle
Additional Information:© 1967 Elsevier Ltd. Received 1 August 1966. Thanks are extended to Mr. Regis B. Kelly and Mr. John Scdat for their constant criticism of this work. This research was supported, in part, by grants RG6965 and GM 13554 from the U.S. Public Health Service.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
U.S. Public Health Service (USPHS)GM 13554
Record Number:CaltechAUTHORS:20160620-070308711
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160620-070308711
Official Citation:Godfrey N. Godson, Robert L. Sinsheimer, The replication of bacteriophage MS2, Journal of Molecular Biology, Volume 23, Issue 3, 1967, Pages 495-521, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(67)80121-9. (http://www.sciencedirect.com/science/article/pii/S0022283667801219)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68500
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:20 Jun 2016 19:20
Last Modified:20 Jun 2016 19:20

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