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Evolutionary Genomics Suggests That CheV Is an Additional Adaptor for Accommodating Specific Chemoreceptors within the Chemotaxis Signaling Complex

Ortega, Davi R. and Zhulin, Igor B. (2016) Evolutionary Genomics Suggests That CheV Is an Additional Adaptor for Accommodating Specific Chemoreceptors within the Chemotaxis Signaling Complex. PLOS Computational Biology, 12 (2). Art. No. e1004723. ISSN 1553-7358. PMCID PMC4742279. http://resolver.caltech.edu/CaltechAUTHORS:20160620-123825694

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Abstract

Escherichia coli and Salmonella enterica are models for many experiments in molecular biology including chemotaxis, and most of the results obtained with one organism have been generalized to another. While most components of the chemotaxis pathway are strongly conserved between the two species, Salmonella genomes contain some chemoreceptors and an additional protein, CheV, that are not found in E. coli. The role of CheV was examined in distantly related species Bacillus subtilis and Helicobacter pylori, but its role in bacterial chemotaxis is still not well understood. We tested a hypothesis that in enterobacteria CheV functions as an additional adaptor linking the CheA kinase to certain types of chemoreceptors that cannot be effectively accommodated by the universal adaptor CheW. Phylogenetic profiling, genomic context and comparative protein sequence analyses suggested that CheV interacts with specific domains of CheA and chemoreceptors from an orthologous group exemplified by the Salmonella McpC protein. Structural consideration of the conservation patterns suggests that CheV and CheW share the same binding spot on the chemoreceptor structure, but have some affinity bias towards chemoreceptors from different orthologous groups. Finally, published experimental results and data newly obtained via comparative genomics support the idea that CheV functions as a “phosphate sink” possibly to off-set the over-stimulation of the kinase by certain types of chemoreceptors. Overall, our results strongly suggest that CheV is an additional adaptor for accommodating specific chemoreceptors within the chemotaxis signaling complex.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1371/journal.pcbi.1004723DOIArticle
http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1004723PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742279/PubMed CentralArticle
ORCID:
AuthorORCID
Ortega, Davi R.0000-0002-8344-2335
Additional Information:© 2016 Ortega, Zhulin. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: September 6, 2015; Accepted: December 29, 2015; Published: February 4, 2016. We thank Joe Falke, Ariane Briegel, Chris Rao, and Tom Shimizu for helpful discussions. Author Contributions: Conceived and designed the experiments: DRO IBZ. Performed the experiments: DRO. Analyzed the data: DRO IBZ. Wrote the paper: DRO IBZ. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported in part by National Institutes of Health grant GM072295. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors have declared that no competing interests exist.
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Funding AgencyGrant Number
NIHGM-072295
PubMed Central ID:PMC4742279
Record Number:CaltechAUTHORS:20160620-123825694
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160620-123825694
Official Citation:Ortega DR, Zhulin IB (2016) Evolutionary Genomics Suggests That CheV Is an Additional Adaptor for Accommodating Specific Chemoreceptors within the Chemotaxis Signaling Complex. PLoS Comput Biol 12(2): e1004723. doi:10.1371/journal.pcbi.1004723
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68531
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:20 Jun 2016 20:03
Last Modified:27 Sep 2017 19:52

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