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A Microfluidic Device for Kinetic Optimization of Protein Crystallization and In Situ Structure Determination

Hansen, Carl L. and Classen, Scott and Berger, James M. and Quake, Stephen R. (2006) A Microfluidic Device for Kinetic Optimization of Protein Crystallization and In Situ Structure Determination. Journal of the American Chemical Society, 128 (10). pp. 3142-3143. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20160620-124352999

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Abstract

The unprecedented economies of scale and unique mass transport properties of microfluidic devices made them viable nano-volume protein crystallization screening platforms. However, realizing the full potential of microfluidic crystallization requires complementary technologies for crystal optimization and harvesting. In this paper, we report a microfluidic device which provides a link between chip-based nanoliter volume crystallization screening and structure analysis through “kinetic optimization” of crystallization reactions and in situ structure determination. Kinetic optimization through systematic variation of reactor geometry and actuation of micromechanical valves is used to screen a large ensemble of kinetic trajectories that are not practical with conventional techniques. Using this device, we demonstrate control over crystal quality, reliable scale-up from nanoliter volume reactions, facile harvesting and cryoprotectant screening, and protein structure determination at atomic resolution from data collected in-chip.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja0576637DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja0576637PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja0576637PublisherSupporting Information
Additional Information:© 2006 American Chemical Society. Received November 10, 2005. Publication Date (Web): February 15, 2006. This work was funded by National Institutes of Health (R01 HG003594, R01-CA77373), the Natural Sciences and Engineering Research Council of Canada (J. Payette Fellowship), the American Cancer Society (PF-03-124-01-GMC), the Mathers Foundation, and the National Cancer Institute (CA92584).
Funders:
Funding AgencyGrant Number
NIHR01 HG003594
NIHR01-CA77373
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
American Cancer SocietyPF-03-124-01-GMC
Mathers FoundationUNSPECIFIED
NIHCA92584
Issue or Number:10
Record Number:CaltechAUTHORS:20160620-124352999
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160620-124352999
Official Citation:A Microfluidic Device for Kinetic Optimization of Protein Crystallization and In Situ Structure Determination Carl L. Hansen, Scott Classen, James M. Berger, and Stephen R. Quake Journal of the American Chemical Society 2006 128 (10), 3142-3143 DOI: 10.1021/ja0576637
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68532
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:20 Jun 2016 20:07
Last Modified:03 Oct 2019 10:14

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