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Sindbis Virus Infection of Chick and Hamster Cells: Synthesis of Virus-Specific Proteins

Strauss, James H., Jr. and Burge, Boyce W. and Darnell, James E. (1969) Sindbis Virus Infection of Chick and Hamster Cells: Synthesis of Virus-Specific Proteins. Virology, 37 (3). pp. 367-376. ISSN 0042-6822. https://resolver.caltech.edu/CaltechAUTHORS:20160620-145903644

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Abstract

In monolayers of chick embryo fibroblasts or BHK cells infected with Sindbis virus in the presence of actinomycin, host protein synthesis is inhibited by at least 80-90% between 2 and 3 hours after infection. Thereafter, Sindbis specified polypeptide chains form 60-70% of total protein synthesis. In particular, synthesis of the membrane protein of the virus constitutes 25% of total protein synthesis. Twelve to 16 polypeptides induced by virus infection have been identified in infected cells by acrylamide gel electrophoresis. The pattern of virus-specified proteins observed depends upon the length of the labeling period, the host cell, and mutations in the virus genome. The molecular weight of the membrane protein of the Sindbis virion has been estimated to be 53,000 and the core protein is estimated to be 30,000 in molecular weight.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0042-6822(69)90220-7DOIArticle
http://www.sciencedirect.com/science/article/pii/0042682269902207PublisherArticle
Additional Information:© 1969 Elsevier Inc. Accepted October 24, 1968. We acknowledge the competent technical assistance of Mrs. Judy Rothberg and of Mr. Robert Tushinski.
Issue or Number:3
Record Number:CaltechAUTHORS:20160620-145903644
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160620-145903644
Official Citation:James H. Strauss, Boyce W. Burge, James E. Darnell, Sindbis virus infection of chick and hamster cells: Synthesis of virus-specific proteins, Virology, Volume 37, Issue 3, 1969, Pages 367-376, ISSN 0042-6822, http://dx.doi.org/10.1016/0042-6822(69)90220-7. (http://www.sciencedirect.com/science/article/pii/0042682269902207)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68544
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:22 Jun 2016 04:45
Last Modified:03 Oct 2019 10:14

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