Strauss, James H., Jr. and Kelly, Regis B. and Sinsheimer, Robert L. (1968) Denaturation of RNA with dimethyl sulfoxide. Biopolymers, 6 (6). pp. 793-807. ISSN 0006-3525. doi:10.1002/bip.1968.360060604. https://resolver.caltech.edu/CaltechAUTHORS:20160620-160329550
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Abstract
The denaturation of single-stranded and double-stranded RNA's in solutions with varying proportions of dimethyl sulfoxide has been followed by changes in absorbancy, optical rotation, and—with a double-stranded form of bacteriophage of MS2 RNA— infectivity for bacterial spheroplasts. By these criteria the RNA's studied, including the synthetic polynucleotide rG:rC, are completely denatured at room temperature in high concentrations of this solvent. In lower concentrations, the T_m of the RNA preparation is decreased only slightly as the dimethyl sulfoxide concentration is raised until a critical concentration is reached. The T_m falls sharply with small further increases in dimethyl sulfoxide concentration. Sedimentation studies can be conducted directly in these media. The determination of sedimentation velocity in 99% dimethyl sulfoxide containing 0.001M EDTA provides a reliable estimate of RNA molecular weights.
Item Type: | Article | |||||||||
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Additional Information: | © 1968 John Wiley & Sons, Inc. | |||||||||
Issue or Number: | 6 | |||||||||
DOI: | 10.1002/bip.1968.360060604 | |||||||||
Record Number: | CaltechAUTHORS:20160620-160329550 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160620-160329550 | |||||||||
Official Citation: | Strauss, J. H., Kelly, R. B. and Sinsheimer, R. L. (1968), Denaturation of RNA with dimethyl sulfoxide. Biopolymers, 6: 793–807. doi: 10.1002/bip.1968.360060604 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 68550 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Donna Wrublewski | |||||||||
Deposited On: | 22 Jun 2016 04:42 | |||||||||
Last Modified: | 11 Nov 2021 04:01 |
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