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Denaturation of RNA with dimethyl sulfoxide

Strauss, James H., Jr. and Kelly, Regis B. and Sinsheimer, Robert L. (1968) Denaturation of RNA with dimethyl sulfoxide. Biopolymers, 6 (6). pp. 793-807. ISSN 0006-3525. https://resolver.caltech.edu/CaltechAUTHORS:20160620-160329550

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Abstract

The denaturation of single-stranded and double-stranded RNA's in solutions with varying proportions of dimethyl sulfoxide has been followed by changes in absorbancy, optical rotation, and—with a double-stranded form of bacteriophage of MS2 RNA— infectivity for bacterial spheroplasts. By these criteria the RNA's studied, including the synthetic polynucleotide rG:rC, are completely denatured at room temperature in high concentrations of this solvent. In lower concentrations, the T_m of the RNA preparation is decreased only slightly as the dimethyl sulfoxide concentration is raised until a critical concentration is reached. The T_m falls sharply with small further increases in dimethyl sulfoxide concentration. Sedimentation studies can be conducted directly in these media. The determination of sedimentation velocity in 99% dimethyl sulfoxide containing 0.001M EDTA provides a reliable estimate of RNA molecular weights.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/bip.1968.360060604DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/bip.1968.360060604/abstractPublisherArticle
Additional Information:© 1968 John Wiley & Sons, Inc.
Issue or Number:6
Record Number:CaltechAUTHORS:20160620-160329550
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160620-160329550
Official Citation:Strauss, J. H., Kelly, R. B. and Sinsheimer, R. L. (1968), Denaturation of RNA with dimethyl sulfoxide. Biopolymers, 6: 793–807. doi: 10.1002/bip.1968.360060604
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68550
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:22 Jun 2016 04:42
Last Modified:03 Oct 2019 10:14

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