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Fluorescence Near-Field Microscopy of DNA at Sub-10 nm Resolution

Ma, Ziyang and Gerton, Jordan M. and Wade, Lawrence A. and Quake, Stephen R. (2006) Fluorescence Near-Field Microscopy of DNA at Sub-10 nm Resolution. Physical Review Letters, 97 (26). Art. No. 260801. ISSN 0031-9007. doi:10.1103/PhysRevLett.97.260801.

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We demonstrate apertureless near-field microscopy of single molecules at sub-10 nm resolution. With a novel phase filter, near-field images of single organic fluorophores were obtained with ~sixfold improvement in the signal-to-noise ratio. The improvement allowed pairs of molecules separated by ~15 nm to be reliably and repeatedly resolved, thus demonstrating the first true Rayleigh resolution test for near-field images of single molecules. The potential of this technique for biological applications was demonstrated with an experiment that measured the helical rise of A-form DNA.

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Additional Information:© 2006 The American Physical Society (Received 21 October 2005; published 27 December 2006) The authors thank G. Lessard for contributions to the development of initial data acquisition and data analysis algorithms. This work was supported by Pharmagenomix Inc. and the NIH Director’s Pioneer program.
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Pharmagenomix Inc.UNSPECIFIED
Issue or Number:26
Record Number:CaltechAUTHORS:MAZprl06
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6859
Deposited By: Archive Administrator
Deposited On:29 Dec 2006
Last Modified:08 Nov 2021 20:37

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