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Investigation of Neovascularization in Three-Dimensional Porous Scaffolds In Vivo by a Combination of Multiscale Photoacoustic Microscopy and Optical Coherence Tomography

Cai, Xin and Zhang, Yu and Li, Li and Choi, Sung-Wook and MacEwan, Matthew R. and Yao, Junjie and Kim, Chulhong and Xia, Younan and Wang, Lihong V. (2013) Investigation of Neovascularization in Three-Dimensional Porous Scaffolds In Vivo by a Combination of Multiscale Photoacoustic Microscopy and Optical Coherence Tomography. Tissue Engineering Part C: Methods, 19 (3). pp. 196-204. ISSN 1937-3384. PMCID PMC3557912. http://resolver.caltech.edu/CaltechAUTHORS:20160622-124239206

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Abstract

It is a grand challenge to visualize and assess in vivo neovascularization in a three-dimensional (3D) scaffold noninvasively, together with high spatial resolution and deep penetration depth. Here we used multiscale photoacoustic microscopy (PAM), including acoustic-resolution PAM (AR-PAM) and optical-resolution PAM (OR-PAM), to chronically monitor neovascularization in an inverse opal scaffold implanted in a mouse model up to 6 weeks by taking advantage of the optical absorption contrast intrinsic to hemoglobin molecules in red blood cells. By combining with optical coherence tomography (OCT) based on optical scattering contrast, we also demonstrated the capability to simultaneously image and analyze the vasculature and the scaffold in the same mouse. The hybrid system containing OR-PAM and OCT offered a fine lateral resolution of ∼5 μm and a penetration depth of ∼1 mm into the scaffold/tissue construct. AR-PAM further extended the penetration depth up to ∼3 mm at a lateral resolution of ∼45 μm. By quantifying the 3D PAM data, we further examined the effect of pore size (200 vs. 80 μm) of a scaffold on neovascularization. The data collected from PAM were consistent with those obtained from traditional invasive, labor-intensive histologic analyses.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1089/ten.tec.2012.0326DOIArticle
http://online.liebertpub.com/doi/abs/10.1089/ten.tec.2012.0326PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557912PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2012 Mary Ann Liebert, Inc. publishers. Received: May 29, 2012; Accepted: July 20, 2012; Online Publication Date: September 10, 2012. We are grateful to Lynnea Brumbaugh for close reading of the manuscript. This work was supported in part by an NIH Director's Pioneer Award (DP1 OD000798) and startup funds from the Washington University in St. Louis (to Y.X.). This work was also sponsored by the NIH grants (R01 EB000712, R01 EB008085, R01 CA140220, R01 CA157277, R01 CA159959, and U54 CA136398, to L.V.W.). Part of the research was performed at the Alafi Neuroimaging Laboratory of the Hope Center for Neurological Disorders, which was supported by the NIH Neuroscience Blueprint Center Core Grant P30 NS057105. Disclosure Statement: L.V.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work. All other authors declare no competing financial interests.
Funders:
Funding AgencyGrant Number
NIHDP1 OD000798
Washington UniversityUNSPECIFIED
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA140220
NIHR01 CA157277
NIHR01 CA159959
NIHU54 CA136398
NIHP30 NS057105
PubMed Central ID:PMC3557912
Record Number:CaltechAUTHORS:20160622-124239206
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160622-124239206
Official Citation:Xin Cai, Yu Zhang, Li Li, Sung-Wook Choi, Matthew R. MacEwan, Junjie Yao, Chulhong Kim, Younan Xia, and Lihong V. Wang. Tissue Engineering Part C: Methods. January 2013, 19(3): 196-204. doi:10.1089/ten.tec.2012.0326
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68590
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Jun 2016 20:08
Last Modified:23 Jun 2016 20:08

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