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Immediate alterations in intestinal oxygen saturation and blood flow after massive small bowel resection as measured by photoacoustic microscopy

Rowland, Kathryn J. and Yao, Junjie and Wang, Lidai and Erwin, Christopher R. and Maslov, Konstantin I. and Wang, Lihong V. and Warner, Brad W. (2012) Immediate alterations in intestinal oxygen saturation and blood flow after massive small bowel resection as measured by photoacoustic microscopy. Journal of Pediatric Surgery, 47 (6). pp. 1143-1149. ISSN 0022-3468. PMCID PMC3377986. doi:10.1016/j.jpedsurg.2012.03.020. https://resolver.caltech.edu/CaltechAUTHORS:20160622-145944485

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Abstract

Purpose: Massive small bowel resection (SBR) results in villus angiogenesis and a critical adaptation response within the remnant bowel. Previous ex vivo studies of intestinal blood flow after SBR are conflicting. We sought to determine the effect of SBR on intestinal hemodynamics using photoacoustic microscopy, a noninvasive, label-free, high-resolution in vivo hybrid imaging modality. Methods: Photoacoustic microscopy was used to image the intestine microvascular system and measure blood flow and oxygen saturation (SO_2) of the terminal mesenteric arteriole and accompanying vein in C57BL6 mice (n = 7) before and immediately after a 50% proximal SBR. A P value of less than .05 was considered significant. Results: Before SBR, arterial and venous SO_2 were similar. Immediately after SBR, the venous SO_2 decreased with an increase in the oxygen extraction fraction. In addition, the arterial and venous blood flow significantly decreased. Conclusion: Massive SBR results in an immediate reduction in intestinal blood flow and increase in tissue oxygen utilization. These physiologic changes are observed throughout the remnant small intestine. The contribution of these early hemodynamic alterations may contribute to the induction of villus angiogenesis and the pathogenesis of normal intestinal adaptation responses.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.jpedsurg.2012.03.020DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022346812002606PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377986PubMed CentralArticle
ORCID:
AuthorORCID
Maslov, Konstantin I.0000-0003-3408-8840
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2012 Elsevier Inc. Received 24 February 2012, Accepted 5 March 2012, Available online 15 June 2012. Wang laboratory research was supported by the National Institutes of Health Grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, R01 CA157277, and 5P60 DK02057933. LVW has a financial interest in Microphotoacoustics, Inc, and Endra, Inc, which, however, did not support this work. Warner laboratory research was support by National Institutes of Health Grants R01 DK059288 (Warner) and T32 CA009621 (Rowland), and the St. Louis Children's Hospital Foundation - Children's Surgical Sciences Institute.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 EB010049
NIHR01 CA157277
NIH5P60 DK02057933
NIHR01 DK059288
NIHT32 CA009621
St. Louis Children's Hospital Foundation-Children's Surgical Sciences InstituteUNSPECIFIED
Subject Keywords:Small bowel resection; SBR; Oxygen saturation; Blood flow; Photoacoustic microscopy
Issue or Number:6
PubMed Central ID:PMC3377986
DOI:10.1016/j.jpedsurg.2012.03.020
Record Number:CaltechAUTHORS:20160622-145944485
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160622-145944485
Official Citation:Kathryn J. Rowland, Junjie Yao, Lidai Wang, Christopher R. Erwin, Konstantin I. Maslov, Lihong V. Wang, Brad W. Warner, Immediate alterations in intestinal oxygen saturation and blood flow after massive small bowel resection as measured by photoacoustic microscopy, Journal of Pediatric Surgery, Volume 47, Issue 6, June 2012, Pages 1143-1149, ISSN 0022-3468, http://dx.doi.org/10.1016/j.jpedsurg.2012.03.020. (http://www.sciencedirect.com/science/article/pii/S0022346812002606)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68606
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Jun 2016 00:07
Last Modified:11 Nov 2021 04:02

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