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Reprogramming of avian neural crest axial identity and cell fate

Simões-Costa, Marcos and Bronner, Marianne E. (2016) Reprogramming of avian neural crest axial identity and cell fate. Science, 352 (6293). pp. 1570-1573. ISSN 0036-8075. PMCID PMC5100669. http://resolver.caltech.edu/CaltechAUTHORS:20160624-140845423

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Abstract

Neural crest populations along the embryonic body axis of vertebrates differ in developmental potential and fate, so that only the cranial neural crest can contribute to the craniofacial skeleton in vivo. We explored the regulatory program that imbues the cranial crest with its specialized features. Using axial-level specific enhancers to isolate and perform genome-wide profiling of the cranial versus trunk neural crest in chick embryos, we identified and characterized regulatory relationships between a set of cranial-specific transcription factors. Introducing components of this circuit into neural crest cells of the trunk alters their identity and endows these cells with the ability to give rise to chondroblasts in vivo. Our results demonstrate that gene regulatory circuits that support the formation of particular neural crest derivatives may be used to reprogram specific neural crest–derived cell types.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.aaf2729DOIArticle
http://science.sciencemag.org/content/352/6293/1570PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100669PubMed CentralArticle
ORCID:
AuthorORCID
Bronner, Marianne E.0000-0003-4274-1862
Additional Information:© 2016 American Association for the Advancement of Science. Received 18 January 2016; accepted 23 May 2016. We thank J. Tan-Cabugao, M. Stone, B. Jun, and D. S. E. Koo for technical assistance. The Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory provided sequencing and bioinformatics support. We are indebted to D. Perez, K. Beadle, and R. Diamond for cell-sorting assistance at the Caltech Flow Cytometry Cell Sorting Facility. We also thank M. Barembaum for the Sox8 and Tfap2b expression constructs. This work was supported by NIH grants DE024157 and HD037105 to M.E.B. M.S.-C. was funded by a fellowship from the Pew Fellows Program in Biomedical Sciences and by NIH grant K99DE024232. The supplementary materials contain additional data.
Funders:
Funding AgencyGrant Number
NIHDE024157
NIHHD037105
Pew FoundationUNSPECIFIED
NIHK99DE024232
PubMed Central ID:PMC5100669
Record Number:CaltechAUTHORS:20160624-140845423
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160624-140845423
Official Citation:Reprogramming of avian neural crest axial identity and cell fate BY MARCOS SIMOES-COSTA, MARIANNE E. BRONNER Science 24 Jun 2016: Vol. 352, Issue 6293, pp. 1570-1573 DOI: 10.1126/science.aaf2729
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68664
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:25 Jun 2016 01:51
Last Modified:18 Jul 2017 21:34

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