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A self-assembled microlensing rotational probe

Brody, James P. and Quake, Stephen R. (1999) A self-assembled microlensing rotational probe. Applied Physics Letters, 74 (1). pp. 144-146. ISSN 0003-6951. doi:10.1063/1.122977.

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A technique to measure microscopic rotational motion is presented. When a small fluorescent polystyrene microsphere is attached to a larger polystyrene microsphere, the larger sphere acts as a lens for the smaller microsphere and provides an optical signal that is a strong function of the azimuthal angle. We demonstrate the technique by measuring the rotational diffusion constant of the microsphere in solutions of varying viscosity and discuss the feasibility of using this probe to measure rotational motion of biological systems.

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Additional Information:©1999 American Institute of Physics. (Received 14 September 1998; accepted 30 October 1998) This work was supported by the Whitaker Foundation and by NSF CAREER Grant No. PHY-9722417.
Subject Keywords:biological techniques; self-assembly; angular measurement; biodiffusion; microlenses; microorganisms; ray tracing; optical microscopy
Issue or Number:1
Record Number:CaltechAUTHORS:BROapl99
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6873
Deposited By: Archive Administrator
Deposited On:30 Dec 2006
Last Modified:08 Nov 2021 20:37

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