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Evolution of hematopoiesis: Three members of the PU.1 transcription factor family in a cartilaginous fish, Raja eglanteria

Anderson, Michele K. and Sun, Xiao and Miracle, Ann L. and Litman, Gary W. and Rothenberg, Ellen V. (2001) Evolution of hematopoiesis: Three members of the PU.1 transcription factor family in a cartilaginous fish, Raja eglanteria. Proceedings of the National Academy of Sciences of the United States of America, 98 (2). pp. 553-558. ISSN 0027-8424. PMCID PMC14625. https://resolver.caltech.edu/CaltechAUTHORS:ANDpnas01.pdf

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Abstract

T lymphocytes and B lymphocytes are present in jawed vertebrates, including cartilaginous fishes, but not in jawless vertebrates or invertebrates. The origins of these lineages may be understood in terms of evolutionary changes in the structure and regulation of transcription factors that control lymphocyte development, such as PU.1. The identification and characterization of three members of the PU.1 family of transcription factors in a cartilaginous fish, Raja eglanteria, are described here. Two of these genes are orthologs of mammalian PU.1 and Spi-C, respectively, whereas the third gene, Spi-D, is a different family member. In addition, a PU.1-like gene has been identified in a jawless vertebrate, Petromyzon marinus (sea lamprey). Both DNA-binding and transactivation domains are highly conserved between mammalian and skate PU.1, in marked contrast to lamprey Spi, in which similarity is evident only in the DNA-binding domain. Phylogenetic analysis of sequence data suggests that the appearance of Spi-C may predate the divergence of the jawed and jawless vertebrates and that Spi-D arose before the divergence of the cartilaginous fish from the lineage leading to the mammals. The tissue-specific expression patterns of skate PU.1 and Spi-C suggest that these genes share regulatory as well as structural properties with their mammalian orthologs.


Item Type:Article
Related URLs:
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC14625/PubMed CentralArticle
ORCID:
AuthorORCID
Rothenberg, Ellen V.0000-0002-3901-347X
Additional Information:© 2001 The National Academy of Sciences. Edited by Max D. Cooper, University of Alabama, Birmingham, AL, and approved November 7, 2000 (received for review October 9, 2000). This paper was submitted directly (Track II) to the PNAS office. Published online before print January 9, 2001, 10.1073/pnas.021478998 Data deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. AF320627, AF320628, and AF320629). We thank Carl Luer and Cathy Walsh of the Mote Marine Laboratory in Sarasota, FL for their valuable assistance in identification and retrieval of hematopoietic skate tissues. We also thank Rashmi Pant for technical assistance. This work was supported by National Aeronautics and Space Administration Grant NAG2-1370 (to M.K.A. and E.V.R.) and National Institutes of Health Grant R37 AI2338 (to G.W.L.). M.K.A. is supported by the Stowers Institute for Medical Research. A.L.M. has been supported in part by the Institute for Biomolecular Science, University of South Florida. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NASANAG2-1370
NIHR37 AI2338
Stowers Institute for Medical ResearchUNSPECIFIED
University of South Florida Institute for Biomolecular ScienceUNSPECIFIED
Issue or Number:2
PubMed Central ID:PMC14625
Record Number:CaltechAUTHORS:ANDpnas01.pdf
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:ANDpnas01.pdf
Alternative URL:http://dx.doi.org/10.1073/pnas.021478998
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:688
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:14 Sep 2005
Last Modified:02 Oct 2019 22:36

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