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Characterization of the Rubella Virus Nonstructural Protease Domain and Its Cleavage Site

Chen, Jun-Ping and Strauss, James H. and Strauss, Ellen G. and Frey, Teryl K. (1996) Characterization of the Rubella Virus Nonstructural Protease Domain and Its Cleavage Site. Journal of Virology, 70 (7). pp. 4707-4713. ISSN 0022-538X. PMCID PMC190407. http://resolver.caltech.edu/CaltechAUTHORS:20160701-144021522

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Abstract

The region of the rubella virus nonstructural open reading frame that contains the papain-like cysteine protease domain and its cleavage site was expressed with a Sindbis virus vector. Cys-1151 has previously been shown to be required for the activity of the protease (L. D. Marr, C.-Y. Wang, and T. K. Frey, Virology 198:586–592, 1994). Here we show that His-1272 is also necessary for protease activity, consistent with the active site of the enzyme being composed of a catalytic dyad consisting of Cys-1151 and His-1272. By means of radiochemical amino acid sequencing, the site in the polyprotein cleaved by the nonstructural protease was found to follow Gly-1300 in the sequence Gly-1299–Gly-1300–Gly-1301. Mutagenesis studies demonstrated that change of Gly-1300 to alanine or valine abrogated cleavage. In contrast, Gly-1299 and Gly-1301 could be changed to alanine with retention of cleavage, but a change to valine abrogated cleavage. Coexpression of a construct that contains a cleavage site mutation (to serve as a protease) together with a construct that contains a protease mutation (to serve as a substrate) failed to reveal trans cleavage. Coexpression of wild-type constructs with protease-mutant constructs also failed to reveal trans cleavage, even after extended in vitro incubation following lysis. These results indicate that the protease functions only in cis, at least under the conditions tested.


Item Type:Article
Related URLs:
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http://jvi.asm.org/content/70/7/4707.longPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC190407/PubMed CentralArticle
Additional Information:© 1996, American Society for Microbiology Received 27 October 1995/Accepted 3 April 1996 We thank Robert Simmons for technical imaging. This work was supported by Public Health Service grants AI 21389 (T.K.F.) and AI 10793 (J.H.S.) from the National Institute of Allergy and Infectious Diseases.
Funders:
Funding AgencyGrant Number
NIHAI 21389
NIHAI 10793
PubMed Central ID:PMC190407
Record Number:CaltechAUTHORS:20160701-144021522
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160701-144021522
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68817
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:05 Jul 2016 03:22
Last Modified:03 Apr 2017 22:31

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