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Recombinational History and Molecular Evolution of Western Equine Encephalomyelitis Complex Alphaviruses

Weaver, Scott C. and Kang, Wenli and Shirako, Yukio and Rümenapf, Tillmann and Strauss, Ellen G. and Strauss, James H. (1997) Recombinational History and Molecular Evolution of Western Equine Encephalomyelitis Complex Alphaviruses. Journal of Virology, 71 (1). pp. 613-623. ISSN 0022-538X. PMCID PMC191092. http://resolver.caltech.edu/CaltechAUTHORS:20160701-151815908

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Abstract

Western equine encephalomyelitis (WEE) virus (Togaviridae: Alphavirus) was shown previously to have arisen by recombination between eastern equine encephalomyelitis (EEE)- and Sindbis-like viruses (C. S. Hahn, S. Lustig, E. G. Strauss, and J. H. Strauss, Proc. Natl. Acad. Sci. USA 85:5997–6001, 1988). We have now examined the recombinational history and evolution of all viruses belonging to the WEE antigenic complex, including the Buggy Creek, Fort Morgan, Highlands J, Sindbis, Babanki, Ockelbo, Kyzylagach, Whataroa, and Aura viruses, using nucleotide sequences derived from representative strains. Two regions of the genome were examined: sequences of 477 nucleotides from the C terminus of the E1 envelope glycoprotein gene which in WEE virus was derived from the Sindbis-like virus parent, and 517 nucleotide sequences at the C terminus of the nsP4 gene which in WEE virus was derived from the EEE-like virus parent. Trees based on the E1 region indicated that all members of the WEE virus complex comprise a monophyletic group. Most closely related to WEE viruses are other New World members of the complex: the Highlands J, Buggy Creek, and Fort Morgan viruses. More distantly related WEE complex viruses included the Old World Sindbis, Babanki, Ockelbo, Kyzylagach, and Whataroa viruses, as well as the New World Aura virus. Detailed analyses of 38 strains of WEE virus revealed at least 4 major lineages; two were represented by isolates from Argentina, one was from Brazil, and a fourth contained isolates from many locations in South and North America as well as Cuba. Trees based on the nsP4 gene indicated that all New World WEE complex viruses except Aura virus are recombinants derived from EEE- and Sindbis-like virus ancestors. In contrast, the Old World members of the WEE complex, as well as Aura virus, did not appear to have recombinant genomes. Using an evolutionary rate estimate (2.8x10^-4) substitutions per nucleotide per year) obtained from E1-3' sequences of WEE viruses, we estimated that the recombination event occurred in the New World 1,300 to 1,900 years ago. This suggests that the alphaviruses originated in the New World a few thousand years ago.


Item Type:Article
Related URLs:
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http://jvi.asm.org/content/71/1/613.longPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC191092/PubMed CentralArticle
Additional Information:Copyright © 1997, American Society for Microbiology Received 22 July 1996/Accepted 27 September 1996 We thank Charles Calisher, Ronald Cheshier, Robert Chiles, James Hardy, Nick Karabatsos, Craig Levy, Robert Shope, and Robert Tesh for providing alphavirus strains. Gabriela Aviles, Charles Calisher, Carl Mitchell, and Marta Sabattini made many helpful suggestions during preparation of the manuscript. The research was supported by grants AI 10984, AI 39508, AI 20612, and AI 10793 from the National Institutes of Health and by the John Sealy Memorial Endowment Fund for Biomedical Research.
Funders:
Funding AgencyGrant Number
NIHAI 10984
NIHAI 39508
NIHAI 20612
NIHAI 10793
John Sealy Memorial Endowment Fund for Biomedical ResearchUNSPECIFIED
PubMed Central ID:PMC191092
Record Number:CaltechAUTHORS:20160701-151815908
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160701-151815908
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68819
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:05 Jul 2016 03:34
Last Modified:05 Jul 2016 03:34

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