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Chronic label-free volumetric photoacoustic microscopy of melanoma cells in three-dimensional porous scaffolds

Zhang, Yu and Cai, Xin and Choi, Sung-Wook and Kim, Chulhong and Wang, Lihong V. and Xia, Younan (2010) Chronic label-free volumetric photoacoustic microscopy of melanoma cells in three-dimensional porous scaffolds. Biomaterials, 31 (33). pp. 8651-8658. ISSN 0142-9612 . PMCID PMC2949455. http://resolver.caltech.edu/CaltechAUTHORS:20160706-133413856

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Abstract

Visualizing cells in three-dimensional (3D) scaffolds has been one of the major challenges in tissue engineering. Most current imaging modalities either suffer from poor penetration depth or require exogenous contrast agents. Here, we demonstrate photoacoustic microscopy (PAM) of the spatial distribution and temporal proliferation of cells inside three-dimensional porous scaffolds with thicknesses over 1 mm. Specifically, we evaluated the effects of seeding and culture methods on the spatial distribution of melanoma cells. Spatial distribution of the cells in the scaffold was well-resolved in PAM images. Moreover, the number of cells in the scaffold was quantitatively measured from the as-obtained volumetric information. The cell proliferation profile obtained from PAM correlated well with what was obtained using the traditional 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.biomaterials.2010.07.089DOIArticle
http://www.sciencedirect.com/science/article/pii/S0142961210009610PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949455PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2010 Elsevier Ltd. Received 22 June 2010; Accepted 23 July 2010; Available online 19 August 2010. This work was supported in part by an NIH Director’s Pioneer Award (DP1 OD000798) and startup funds from Washington University in St. Louis (to Y.X.). This work was also sponsored by NIH grants (R01 EB000712, R01 NS46214, R01 EB008085, and U54 CA136398, to L.V.W.). Part of the work was performed at the Nano Research Facility (NRF), a member of the National Nanotechnology Infrastructure Network (NNIN), which is supported by the NSF under award ECS-0335765. L.V.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHDP1 OD000798
Washington UniversityUNSPECIFIED
NIHR01 EB000712
NIHR01 NS46214
NIHR01 EB008085
NIHU54 CA136398
NSFECS-0335765
Subject Keywords:Inverse opal scaffolds; Photoacoustic microscopy; Melanoma; Tissue engineering; Biomedical imaging
PubMed Central ID:PMC2949455
Record Number:CaltechAUTHORS:20160706-133413856
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160706-133413856
Official Citation:Yu Zhang, Xin Cai, Sung-Wook Choi, Chulhong Kim, Lihong V. Wang, Younan Xia, Chronic label-free volumetric photoacoustic microscopy of melanoma cells in three-dimensional porous Scaffolds, Biomaterials, Volume 31, Issue 33, November 2010, Pages 8651-8658, ISSN 0142-9612, http://dx.doi.org/10.1016/j.biomaterials.2010.07.089. (http://www.sciencedirect.com/science/article/pii/S0142961210009610)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68862
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Jul 2016 03:29
Last Modified:07 Jul 2016 03:29

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