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Characterization of the intergenic RNA profile at abdominal-A and Abdominal-B in the Drosophila bithorax complex

Bae, Esther and Calhoun, Vincent C. and Levine, Michael and Lewis, Edward B. and Drewell, Robert A. (2002) Characterization of the intergenic RNA profile at abdominal-A and Abdominal-B in the Drosophila bithorax complex. Proceedings of the National Academy of Sciences of the United States of America, 99 (26). pp. 16847-16852. ISSN 0027-8424. PMCID PMC139232. doi:10.1073/pnas.222671299.

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The correct spatial expression of two Drosophila bithorax complex (BX-C) genes, abdominal-A (abdA) and Abdominal-B (AbdB), is dependent on the 100-kb intergenic infraabdominal (iab) region. The iab region is known to contain a number of different domains (iab2 through iab8) that harbor cis-regulatory elements responsible for directing expression of abdA and AbdB in the second through eighth abdominal segments. Here, we use in situ hybridization to perform high-resolution mapping of the transcriptional activity in the iab control regions. We show that transcription of the control regions themselves is abundant and precedes activation of the abdA and AbdB genes. As with the homeotic genes of the BX-C, the transcription patterns of the RNAs from the iab control regions demonstrate colinearity with the sequence of the iab regions along the chromosome and the domains in the embryo under the control of the specific iab regions. These observations suggest that the intergenic RNAs may play a role in initiating cis regulation at the BX-C early in development.

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Additional Information:© 2002 by The National Academy of Sciences. Contributed by Edward B. Lewis and approved November 4, 2002. Published online before print December 12, 2002, 10.1073/pnas.222671299 We thank Katharine Arney, Andrew Dowsett, and Joanne Topol for comments on the manuscript and Mark Stapleton for providing the engrailed probe. This work was supported by grants from the National Institutes of Health to M.L. (GM34431) and E.B.L. (HD06331). R.A.D. was funded by a Wellcome Trust Prize Traveling Fellowship.
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Subject Keywords:bithorax complex, anteroposterior, abdA promoter-proximal probe, AbdB promoter-proximal probe
Issue or Number:26
PubMed Central ID:PMC139232
Record Number:CaltechAUTHORS:BAEpnas02
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:689
Deposited By: Archive Administrator
Deposited On:14 Sep 2005
Last Modified:08 Nov 2021 19:04

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