A Caltech Library Service

ZOom^3: A three-dimensional super-resolution technique to map brain connectivity from millimeters to molecules

Miller, Carol and Sepehrband, Farshid and Williams, Celia and Talishinsky, Alexander and Mehta, Shagun and Bienkowski, Michael and Gonzalez-Zacarias, Clio and Barnes, Samuel and Jacobs, Russell and Toga, Arthur and Dong, Hongwei and Clark, Kristi (2016) ZOom^3: A three-dimensional super-resolution technique to map brain connectivity from millimeters to molecules. Journal of Neuropathology and Experimental Neurology, 75 (6). pp. 567-616. ISSN 0022-3069.

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item:


In tauopathies such as Alzheimer’s disease (AD), protein aggregates comprised of neurofibrillary tangles, are formed which may potentially impede axonal transport. Misfolded aggregates serve as templates for non-aggregated isoforms. In AD, these are initially intraneuronal fibrils composed of tau and of phosphotau epitopes, as observed by immunohistochemistry. If released at an impaired presynaptic ending, misfolded tau may be picked up by the postsynaptic dendritic. Early in the disease, tau aggregates are present in limbic regions, and later progress to the neocortex, including association areas. In hippocampal subregions, the CLARITY method provides sufficient volume and transparency for immunohistochemical characterization of local connections and for coherent mapping, along with defining neurospecificity. We now show the neuroanatomy and connectivity of the human hippocampus at an unprecedented level of spatial resolution integrating two state-of-the-art techniques: ex vivo high-field diffusion MRI and three-dimensional, two-photon microscopy of CLARITY-processed postmortem human hippocampal tissue. We perform both techniques in the same sample and demonstrate how to co-register the two data types that are spatially aligned so that data from each methodology are superimposed. For the first time, it is now possible with this new methodology called ZOom3, to superimpose cellular and molecular mechanisms that shape connectivity at the micron level with the systems level connectivity patterns observed at the millimeter level within the postmortem human brain. The strength of this method is that individual axons can be resolved microscopically, up to a depth of 8mm, and their trajectory can be mapped directly to the neuroimaging data. This technique will facilitate the future development of in vivo neuroimaging correlation of molecular biomarkers.

Item Type:Article
Related URLs:
URLURL TypeDescription
Barnes, Samuel0000-0002-1065-8442
Jacobs, Russell0000-0002-1382-8486
Additional Information:© 2016 American Association of Neuropathologists First published online: 25 May 2016.
Issue or Number:6
Record Number:CaltechAUTHORS:20160708-070229463
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68901
Deposited By: Ruth Sustaita
Deposited On:09 Jul 2016 04:45
Last Modified:09 Mar 2020 13:18

Repository Staff Only: item control page