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Ribosomal proteins produced in excess are degraded by the ubiquitin-proteasome system

Sung, Min-Kyung and Reitsma, Justin M. and Sweredoski, Michael J. and Hess, Sonja and Deshaies, Raymond J. (2016) Ribosomal proteins produced in excess are degraded by the ubiquitin-proteasome system. Molecular Biology of the Cell, 27 (17). pp. 2642-2652. ISSN 1059-1524. PMCID PMC5007085. https://resolver.caltech.edu/CaltechAUTHORS:20160708-082549598

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Abstract

Ribosome assembly is an essential process that consumes prodigious quantities of cellular resources. Ribosomal proteins cannot be overproduced in Saccharomyces cerevisiae because the excess proteins are rapidly degraded. However, the responsible quality-control (QC) mechanisms remain poorly characterized. Here, we demonstrate that overexpression of multiple proteins of the small and large yeast ribosomal subunits is suppressed. Rpl26 overexpressed from a plasmid can be detected in the nucleolus and nucleoplasm, but it largely fails to assemble into ribosomes and is rapidly degraded. However, if the endogenous RPL26 loci are deleted, plasmid-encoded Rpl26 assembles into ribosomes and localizes to the cytosol. Chemical and genetic perturbation studies indicate that overexpressed ribosomal proteins are degraded by the ubiquitin-proteasome system, and not by autophagy. Inhibition of the proteasome led to accumulation of multiple endogenous ribosomal proteins in insoluble aggregates, consistent with the operation of this QC mechanism in the absence of ribosomal protein overexpression. Our studies reveal that ribosomal proteins that fail to assemble into ribosomes are rapidly distinguished from their assembled counterparts and are ubiquitinated and degraded within the nuclear compartment.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1091/mbc.E16-05-0290DOIArticle
http://www.molbiolcell.org/content/27/17/2642.longPublisherArticle
http://www.molbiolcell.org/content/27/17/2642/suppl/DC1PublisherSupplemental Materials
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007085/PubMed CentralArticle
ORCID:
AuthorORCID
Sweredoski, Michael J.0000-0003-0878-3831
Hess, Sonja0000-0002-5904-9816
Deshaies, Raymond J.0000-0002-3671-9354
Additional Information:© 2016 Sung et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). Submitted May 10, 2016; Revised June 24, 2016; Accepted June 30, 2016; Published online before print July 6, 2016. We are grateful to members of the Deshaies’ laboratory for helpful advice and Rati Verma for critical reading of this manuscript and numerous helpful suggestions regarding biochemical experiments. We thank Jonathan Warner for generously providing reagents and helpful discussions. This work was supported by the Gordon and Betty Moore Foundation, through Grant GBMF775 and the Beckman Institute (to S.H.) and NIH Grant F32 GM112308 (to J.R.). R.J.D. is an Investigator of the HHMI and this work was supported by HHMI.
Funders:
Funding AgencyGrant Number
Gordon and Betty Moore FoundationGBMF775
Caltech Beckman InstituteUNSPECIFIED
NIH Postdoctoral FellowshipF32 GM112308
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:17
PubMed Central ID:PMC5007085
Record Number:CaltechAUTHORS:20160708-082549598
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160708-082549598
Official Citation:Min-Kyung Sung, Justin M. Reitsma, Michael J. Sweredoski, Sonja Hess, and Raymond J. Deshaies A Highlights from MBoC Selection: Ribosomal proteins produced in excess are degraded by the ubiquitin–proteasome system Mol. Biol. Cell 2016 27:17 2642-2652; First Published on July 6, 2016; doi:10.1091/mbc.E16-05-0290
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:68909
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Jul 2016 00:00
Last Modified:03 Oct 2019 10:17

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