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Calibration-free structured-illumination photoacoustic flowgraphy of transverse flow in scattering media

Yao, Junjie and Gilson, Rebecca C. and Maslov, Konstantin I. and Wang, Lidai and Wang, Lihong V. (2014) Calibration-free structured-illumination photoacoustic flowgraphy of transverse flow in scattering media. Journal of Biomedical Optics, 19 (4). Art. No. 046007. ISSN 1083-3668. PMCID PMC3980702. doi:10.1117/1.JBO.19.4.046007.

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We propose a calibration-free photoacoustic (PA) method for transverse flow measurements. In this method, a pulsed periodically structured (i.e., grating patterned) optical beam is used to illuminate flowing absorptive particles in an optically scattering medium. The PA signal amplitudes measured over consecutive laser pulses carry an imprint of the illumination structure. The modulation frequency of the imprint is proportional to the component of the flow speed projected onto the normal axis of the striped illumination pattern. This method can tolerate high particle density, and is insensitive to the particle size, thus calibration-free. Bovine blood and microsphere phantoms were used to validate the proposed method. Blood flow in a mouse ear was measured in vivo as well.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Maslov, Konstantin I.0000-0003-3408-8840
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2014 SPIE. Paper 130871R received Dec. 5, 2013; revised manuscript received Feb. 27, 2014; accepted for publication Mar. 21, 2014; published online Apr. 9, 2014. The authors would like to thank Professor James Ballard for manuscript editing. We also appreciate technical assistance and useful discussions with Arie Krumholz, Yong Zhou Liang S. Gao, and Yan Liu. This research was supported by the National Institutes of Health (Grant Nos. DP1 EB016986 (NIH Director’s Pioneer Award), R01 EB016963, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, and R01 CA159959. L.V.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 EB016963
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 CA157277
NIHR01 CA159959
Subject Keywords:photoacoustic flowgraphy; structured illumination; frequency shift; particle size independent
Issue or Number:4
PubMed Central ID:PMC3980702
Record Number:CaltechAUTHORS:20160713-123826742
Persistent URL:
Official Citation:Yao J, Gilson RC, Maslov KI, Wang L, Wang LV; Calibration-free structured-illumination photoacoustic flowgraphy of transverse flow in scattering media. J. Biomed. Opt. 0001;19(4):046007. doi:10.1117/1.JBO.19.4.046007
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69005
Deposited By: Tony Diaz
Deposited On:27 Jul 2016 16:18
Last Modified:11 Nov 2021 04:07

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