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Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells

Mahata, Sushil K. and Zheng, Hong and Mahata, Sumana and Liu, Xuefei and Patel, Kaushik P. (2016) Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells. Journal of Endocrinology, 230 (3). pp. 309-323. ISSN 0022-0795. PMCID PMC4980258. https://resolver.caltech.edu/CaltechAUTHORS:20160718-091553977

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Abstract

One of the key mechanisms involved in sympathoexcitation in chronic heart failure (HF) is the activation of the adrenal glands. Impact of the elevated catecholamines on the hemodynamic parameters has been previously demonstrated. However, studies linking the structural effects of such overactivation with secretory performance and cell metabolism in the adrenomedullary chromaffin cells in vivo have not been previously reported. In this study, HF was induced in male Sprague-Dawley rats by ligation of the left coronary artery. Five weeks after surgery, cardiac function was assessed by ventricular hemodynamics. HF rats showed increased adrenal weight and adrenal catecholamine levels (norepinephrine, epinephrine and dopamine) compared with sham-operated rats. Rats with HF demonstrated increased small synaptic and dense core vesicle in splanchnic–adrenal synapses indicating trans-synaptic activation of catecholamine biosynthetic enzymes, increased endoplasmic reticulum and Golgi lumen width to meet the demand of increased catecholamine synthesis and release, and more mitochondria with dilated cristae and glycogen to accommodate for the increased energy demand for the increased biogenesis and exocytosis of catecholamines from the adrenal medulla. These findings suggest that increased trans-synaptic activation of the chromaffin cells within the adrenal medulla may lead to increased catecholamines in the circulation which in turn contributes to the enhanced neurohumoral drive, providing a unique mechanistic insight for enhanced catecholamine levels in plasma commonly observed in chronic HF condition.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1530/JOE-16-0146DOIArticle
http://joe.endocrinology-journals.org/content/230/3/309PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980258/PubMed CentralArticle
Additional Information:© 2016 by the Society for Endocrinology. This work is licensed under a Creative Commons Attribution 3.0 Unported License. Received in final form 22 June 2016; Accepted 24 June 2016. Published online before print July 8, 2016. Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported. Funding: This work was supported by the National Institutes of Health grants R56 HL124104 and P01 HL62222.
Funders:
Funding AgencyGrant Number
NIHR56 HL124104
NIHP01 HL62222
Subject Keywords:catecholamines; chromaffin cells; heart failure; sympathetic activation
Issue or Number:3
PubMed Central ID:PMC4980258
Record Number:CaltechAUTHORS:20160718-091553977
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160718-091553977
Official Citation:Sushil K Mahata, Hong Zheng, Sumana Mahata, Xuefei Liu, and Kaushik P Patel Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells J Endocrinol 230 (3) 309-323, doi: 10.1530/JOE-16-0146 First published online 8 July 2016
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69085
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Jul 2016 17:31
Last Modified:03 Oct 2019 10:18

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