DePinho, Ronald and Kruger, Kelly and Andrews, Nancy and Lutzker, Stuart and Baltimore, David and Alt, Frederick W. (1984) Molecular basis of heavy-chain class switching and switch region deletion in an Abelson virus-transformed cell line. Molecular and Cellular Biology, 4 (12). pp. 2905-2910. ISSN 0270-7306. https://resolver.caltech.edu/CaltechAUTHORS:DEPmcb84
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Abstract
We demonstrated that a subclone of an Abelson murine leukemia virus-transformed B-lymphoid cell line switched from mu to gamma 2b expression in vitro, by the classical recombination-deletion mechanism. In this line, the expressed VHDJH region and the C gamma 2b constant region gene were juxtaposed by a recombination event which linked the highly repetitive portions of the S mu and S gama 2b regions and resulted in the loss of the C mu gene from the intervening region. An additional recombination event in this subclone involved an internal deletion in the S mu region of the expressed (switched) allele. One end of this deletion occurred very close to the switch recombination point. Despite the recombination-deletion mechanism of switching, the gamma 2b-producing line retained two copies of the C mu gene and two copies of the sequence just 5' to the S gamma 2b recombination point. The possible significance of the retention of these sequences to the mechanism of class switching is discussed.
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Additional Information: | Copyright © 1984 by the American Society for Microbiology. Received 13 June 1984/Accepted 25 September 1984 We thank K. Marcu for providing the SL1 clone from which the 5'- and 3'-Sγ2b probes were derived and K. Blackwell for critically reading this manuscript. This work was supported by American Cancer Society grants NP-393 (F.W.A.) and MV-34M (D.B.). F.W.A. is a recipient of an Irma T. Hirschl Career Scientist Award. | ||||||
Issue or Number: | 12 | ||||||
Record Number: | CaltechAUTHORS:DEPmcb84 | ||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:DEPmcb84 | ||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
ID Code: | 6913 | ||||||
Collection: | CaltechAUTHORS | ||||||
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Deposited On: | 02 Jan 2007 | ||||||
Last Modified: | 02 Oct 2019 23:37 |
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