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Non-Invasive and In Situ Characterization of the Degradation of Biomaterial Scaffolds by Volumetric Photoacoustic Microscopy

Zhang, Yu Shrike and Cai, Xin and Yao, Junjie and Xing, Wenxin and Wang, Lihong V. and Xia, Younan (2014) Non-Invasive and In Situ Characterization of the Degradation of Biomaterial Scaffolds by Volumetric Photoacoustic Microscopy. Angewandte Chemie International Edition, 53 (1). pp. 184-188. ISSN 1433-7851. PMCID PMC3894115. http://resolver.caltech.edu/CaltechAUTHORS:20160720-123855323

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Abstract

Degradation is among the most important properties of biomaterial scaffolds, which are indispensable for regenerative medicine. The currently used method relies on the measurement of mass loss across different samples and cannot track the degradation of an individual scaffold in situ. Here we report, for the first time, the use of multiscale photoacoustic microscopy to non-invasively monitor the degradation of an individual scaffold. We could observe alterations to the morphology and structure of a scaffold at high spatial resolution and deep penetration, and more significantly, quantify the degradation of an individual scaffold as a function of time, both in vitro and in vivo. In addition, the remodeling of vasculature inside a scaffold can be visualized simultaneously using a dual-wavelength scanning mode in a label-free manner. This optoacoustic method can be used to monitor the degradation of individual scaffolds, offering a new approach to non-invasively analyze and quantify biomaterial–tissue interactions in conjunction with the assessment of in vivo vascular parameters.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/anie.201306282DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/anie.201306282/abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3894115PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2014 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: July 19, 2013; Revised: September 12, 2013; Published online: October 15, 2013. We thank Prof. James Ballard for careful reading of the manuscript. This work was supported by an NIH Director’s Pioneer Award (DP1 D000798) and startup funds from Washington University in St. Louis and Georgia Institute of Technology (to Y.X.). This work was also sponsored by NIH grants (R01 EB000712, R01 EB008085, R01 CA140220, R01 CA157277, R01 CA159959, and U54 CA136398, to L.V.W.).
Funders:
Funding AgencyGrant Number
NIHDP1 OD000798
Washington UniversityUNSPECIFIED
Georgia Institute of TechnologyUNSPECIFIED
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA140220
NIHR01 CA157277
NIHR01 CA159959
NIHU54 CA136398
Subject Keywords:blood vessels; inverse opal scaffolds; MTT formazan; photoacoustic imaging; regenerative medicine
PubMed Central ID:PMC3894115
Record Number:CaltechAUTHORS:20160720-123855323
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160720-123855323
Official Citation:Zhang, Y. S., Cai, X., Yao, J., Xing, W., Wang, L. V. and Xia, Y. (2014), Non-Invasive and In Situ Characterization of the Degradation of Biomaterial Scaffolds by Volumetric Photoacoustic Microscopy. Angew. Chem. Int. Ed., 53: 184–188. doi:10.1002/anie.201306282
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69134
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Jul 2016 20:23
Last Modified:26 Jul 2016 20:23

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