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Optical-Resolution Photoacoustic Microscopy for Volumetric and Spectral Analysis of Histological and Immunochemical Samples

Zhang, Yu Shrike and Yao, Junjie and Zhang, Chi and Li, Lei and Wang, Lihong V. and Xia, Younan (2014) Optical-Resolution Photoacoustic Microscopy for Volumetric and Spectral Analysis of Histological and Immunochemical Samples. Angewandte Chemie International Edition, 53 (31). pp. 8099-8103. ISSN 1433-7851. PMCID PMC4116434. http://resolver.caltech.edu/CaltechAUTHORS:20160720-132527952

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Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) is an imaging modality with superb penetration depth and excellent absorption contrast. Here we demonstrate, for the first time, that this technique can advance quantitative analysis of conventional chromogenic histochemistry. Because OR-PAM can quantify the absorption contrast at different wavelengths, it is feasible to spectrally resolve the specific biomolecules involved in a staining color. Furthermore, the tomographic capability of OR-PAM allows for noninvasive volumetric imaging of a thick sample without microtoming it. By immunostaining the sample with different chromogenic agents, we further demonstrated the ability of OR-PAM to resolve different types of cells in a coculture sample with imaging depths up to 1 mm. Taken together, the integration of OR-PAM with (immuno)histochemistry offers a simple and versatile technique with broad applications in cell biology, pathology, tissue engineering, and related biomedical studies.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/anie.201403812DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/anie.201403812/abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4116434PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2014 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: March 28, 2014; Published online: June 24, 2014. This work was supported by NIH grants DP1 OD000798 (NIH Director’s Pioneer Award) to Y.X., and DP1 EB016986 (NIH Director’s Pioneer Award), R01 CA186567 (NIH Director’s Transformative Research Award), and R01 CA159959 to L.V.W. L.V.W. has a financial interest in Endra, Inc., and Microphotoacoustics, Inc., which did not support this work. Part of the research was performed at the Alafi Neuroimaging Laboratory, the Hope Center for Neurological Disorders in Washington University School of Medicine, which is supported by the NIH Neuroscience Blueprint Center Core Grant P30 NS057105.
Funders:
Funding AgencyGrant Number
NIHDP1 OD000798
NIHDP1 EB016986
NIHR01 CA186567
NIHR01 CA159959
NIHP30 NS057105
PubMed Central ID:PMC4116434
Record Number:CaltechAUTHORS:20160720-132527952
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160720-132527952
Official Citation:Zhang, Y. S., Yao, J., Zhang, C., Li, L., Wang, L. V. and Xia, Y. (2014), Optical-Resolution Photoacoustic Microscopy for Volumetric and Spectral Analysis of Histological and Immunochemical Samples. Angew. Chem. Int. Ed., 53: 8099–8103. doi:10.1002/anie.201403812
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69136
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:25 Jul 2016 22:44
Last Modified:25 Jul 2016 22:44

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