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Photoacoustic lymphatic imaging with high spatial-temporal resolution

Martel, Catherine and Yao, Junjie and Huang, Chih-Hsien and Zou, Jun and Randolph, Gwendalyn J. and Wang, Lihong V. (2014) Photoacoustic lymphatic imaging with high spatial-temporal resolution. Journal of Biomedical Optics, 19 (11). Art. No. 116009. ISSN 1083-3668. PMCID PMC4407768.

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Despite its critical function in coordinating the egress of inflammatory and immune cells out of tissues and maintaining fluid balance, the causative role of lymphatic network dysfunction in pathological settings is still understudied. Engineered-animal models and better noninvasive high spatial-temporal resolution imaging techniques in both preclinical and clinical studies will help to improve our understanding of different lymphatic-related pathologic disorders. Our aim was to take advantage of our newly optimized noninvasive wide-field fast-scanning photoacoustic (PA) microcopy system to coordinately image the lymphatic vasculature and its flow dynamics, while maintaining high resolution and detection sensitivity. Here, by combining the optical-resolution PA microscopy with a fast-scanning water-immersible microelectromechanical system scanning mirror, we have imaged the lymph dynamics over a large field-of-view, with high spatial resolution and advanced detection sensitivity. Depending on the application, lymphatic vessels (LV) were spectrally or temporally differentiated from blood vessels. Validation experiments were performed on phantoms and in vivo to identify the LV. Lymphatic flow dynamics in nonpathological and pathological conditions were also visualized. These results indicate that our newly developed PA microscopy is a promising tool for lymphatic-related biological research.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Zou, Jun0000-0002-9543-6135
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2014 SPIE. Paper 140140RR received Mar. 4, 2014; revised manuscript received Sep. 2, 2014; accepted for publication Oct. 14, 2014; published online Nov. 19, 2014. We thank the manuscript editing and technical support from Prof. James Ballard, Dr. Chun-Cheng Chen, and Prof. William Chapman. This work was sponsored by NIH grants DP1 EB016986 (NIH Director’s Pioneer Award), R01 CA186567 (NIH Director’s Transformative Research Award), R01 CA134539, and R01 CA159959 (to L. V. Wang), R01 HL-096539 (to G.J. Randolph) and NSF grant CMMI-1131758 (to J. Zou). L. V. Wang has a financial interest in Endra, Inc., and Microphotoacoustics, Inc., which, however, did not support this work. Other authors have no conflicts of interest to declare.
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIHR01 CA134539
NIHR01 CA159959
NIHR01 HL-096539
Subject Keywords:photoacoustic microscopy; high-speed imaging; lymphatic vessel; lymphatic dynamics
Issue or Number:11
PubMed Central ID:PMC4407768
Record Number:CaltechAUTHORS:20160720-143115806
Persistent URL:
Official Citation:Martel C, Yao J, Huang C, Zou J, Randolph GJ, Wang LV; Photoacoustic lymphatic imaging with high spatial-temporal resolution. J. Biomed. Opt. 0001;19(11):116009. doi:10.1117/1.JBO.19.11.116009
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69140
Deposited By: Tony Diaz
Deposited On:25 Jul 2016 22:38
Last Modified:09 Mar 2020 13:19

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