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Radioactive ^(198)Au-Doped Nanostructures with Different Shapes for In Vivo Analyses of Their Biodistribution, Tumor Uptake, and Intratumoral Distribution

Black, Kvar C. L. and Wang, Yucai and Luehmann, Hannah P. and Cai, Xin and Xing, Wenxin and Pang, Bo and Zhao, Yongfeng and Cutler, Cathy S. and Wang, Lihong V. and Liu, Yongjian and Xia, Younan (2014) Radioactive ^(198)Au-Doped Nanostructures with Different Shapes for In Vivo Analyses of Their Biodistribution, Tumor Uptake, and Intratumoral Distribution. ACS Nano, 8 (5). pp. 4385-4394. ISSN 1936-0851. PMCID PMC4358630. https://resolver.caltech.edu/CaltechAUTHORS:20160721-081558591

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Abstract

With Au nanocages as an example, we recently demonstrated that radioactive ^(198)Au could be incorporated into the crystal lattice of Au nanostructures for simple and reliable quantification of their in vivo biodistribution by measuring the γ radiation from ^(198)Au decay and for optical imaging by detecting the Cerenkov radiation. Here we extend the capability of this strategy to synthesize radioactive ^(198)Au nanostructures with a similar size but different shapes and then compare their biodistribution, tumor uptake, and intratumoral distribution using a murine EMT6 breast cancer model. Specifically, we investigated Au nanospheres, nanodisks, nanorods, and cubic nanocages. After PEGylation, an aqueous suspension of the radioactive Au nanostructures was injected into a tumor-bearing mouse intravenously, and their biodistribution was measured from the γ radiation while their tumor uptake was directly imaged using the Cerenkov radiation. Significantly higher tumor uptake was observed for the Au nanospheres and nanodisks relative to the Au nanorods and nanocages at 24 h postinjection. Furthermore, autoradiographic imaging was performed on thin slices of the tumor after excision to resolve the intratumoral distributions of the nanostructures. While both the Au nanospheres and nanodisks were only observed on the surfaces of the tumors, the Au nanorods and nanocages were distributed throughout the tumors.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/nn406258mDOIArticle
http://pubs.acs.org/doi/abs/10.1021/nn406258mPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/nn406258mPublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358630PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2014 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Received for review December 5, 2013 and accepted April 25, 2014. Publication Date (Web): April 25, 2014. This work was supported by a grant from the NCI (R01 CA138527) and startup funds from Georgia Institute of Technology. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
NIHR01 CA138527
Georgia Institute of TechnologyUNSPECIFIED
Subject Keywords:biodistribution; Cerenkov imaging; intratumoral distribution; radioactive gold nanostructures; shape dependence; tumor uptake
Issue or Number:5
PubMed Central ID:PMC4358630
Record Number:CaltechAUTHORS:20160721-081558591
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160721-081558591
Official Citation:Radioactive 198Au-Doped Nanostructures with Different Shapes for In Vivo Analyses of Their Biodistribution, Tumor Uptake, and Intratumoral Distribution Kvar C. L. Black, Yucai Wang, Hannah P. Luehmann, Xin Cai, Wenxin Xing, Bo Pang, Yongfeng Zhao, Cathy S. Cutler, Lihong V. Wang, Yongjian Liu, and Younan Xia ACS Nano 2014 8 (5), 4385-4394 DOI: 10.1021/nn406258m
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69146
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:25 Jul 2016 22:23
Last Modified:03 Oct 2019 10:19

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