CaltechAUTHORS
  A Caltech Library Service

Assignment of the receptor for ecotropic murine leukemia virus to mouse chromosome 5

Ruddle, Nancy H. and Conta, Barbara S. and Leinwand, Leslie and Kozak, Christine and Ruddle, Frank and Besmer, Peter and Baltimore, David (1978) Assignment of the receptor for ecotropic murine leukemia virus to mouse chromosome 5. Journal of Experimental Medicine, 148 (2). pp. 451-465. ISSN 0022-1007. https://resolver.caltech.edu/CaltechAUTHORS:RUDjem78

[img]
Preview
PDF
See Usage Policy.

1495Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:RUDjem78

Abstract

The gene for the receptor for ecotropic murine leukemia virus (Rev) has been assigned to mouse chromosome 5. This determination was made possible by an analysis of somatic cell hybrids between mouse and Chinese hamster cells. The parents of these hybrids were A/HeJ or Mus poschiavinus peritoneal exudate cells or BALB/c primary embryo fibroblasts and E36, a Chinese hamster lung fibroblast deficient in hypoxanthine guanine phosphoribosyltransferase. Segregation of mouse chromosomes in these hybrids was analyzed by chromosome banding and isozyme expression. Cells were tested for their ability to absorb and replicate vesicular stomatitis virus (murine leukemia virus [MuLV]) pseudotype particles and ecotropic MuLV as measured by the XC test. The presence of chromosome 5 was essential for receptor expression as determined by three statistical procedures. Segregation of the receptor for ecotropic murine leukemia virus was also followed in two series of subclones. In both, receptor expression was syntenic with phosphoglucomutase-1, an isozyme which has been mapped to mouse chromosome 5.


Item Type:Article
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:Copyright © 1978 by Rockefeller University Press Received for publication 19 April 1978. We thank Rita Freimanis, Daniel Clark, and Elizabeth Nichols for technical assistance. [N.H.R. was] [s]Supported by grants NCI CA 21333, NCI CA 16038, and ACS IM 85. [B.S.C. was] [s]upported by the Interdisciplinary Immunology Training Program AI-07019. [F.R. was the] [r]ecipient of grants NIH GM 09966 and NCI CP VO 7100-54. [D.B. was the] [r]ecipient of grants NCI CA-14051 and ACS VC-41; an American Cancer Society Research Professor.
Issue or Number:2
Record Number:CaltechAUTHORS:RUDjem78
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:RUDjem78
Alternative URL:http://www.jem.org/cgi/content/abstract/148/2/451
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6923
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:02 Jan 2007
Last Modified:02 Oct 2019 23:37

Repository Staff Only: item control page