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Deep-tissue photoacoustic tomography of Förster resonance energy transfer

Wang, Yu and Xia, Jun and Wang, Lihong V. (2013) Deep-tissue photoacoustic tomography of Förster resonance energy transfer. Journal of Biomedical Optics, 18 (10). Art. No. 101316. ISSN 1083-3668. PMCID PMC3719951. doi:10.1117/1.JBO.18.10.101316.

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Förster resonance energy transfer (FRET) is a distance-dependent process that transfers excited state energy from a donor molecule to an acceptor molecule without the emission of a photon. The FRET rate is determined by the proximity between the donor and the acceptor molecules; it becomes significant only when the proximity is within several nanometers. Therefore, FRET has been applied to visualize interactions and conformational changes of biomolecules, such as proteins, lipids, and nucleic acids that cannot be resolved by optical microscopy. Here, we report photoacoustic tomography of FRET efficiency at a 1-cm depth in chicken breast tissue, whereas conventional high-resolution fluorescence imaging is limited to < 0.1  cm. Photoacoustic tomography is expected to facilitate the examination of FRET phenomena in living organisms.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Wang, Yu0000-0003-3589-9274
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2013 SPIE. Paper 130171SSR received Mar. 25, 2013; revised manuscript received Jul. 1, 2013; accepted for publication Jul. 3, 2013; published online Jul. 23, 2013. The authors appreciate the close reading of the manuscript by Profs. James Ballard and Lynnea Brumbaugh. They also thank Alejandro Garcia-Uribe for helping with data processing and manuscript preparation. This work was sponsored in part by National Institutes of Health grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, R01 CA159959, and DP1 EB016986 (NIH Director’s Pioneer Award). L. V.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 CA157277
NIHR01 CA159959
NIHDP1 EB016986
Subject Keywords:photoacoustic tomography; Förster resonance energy transfer; deep imaging; Förster resonance energy transfer efficiency
Issue or Number:10
PubMed Central ID:PMC3719951
Record Number:CaltechAUTHORS:20160727-092516000
Persistent URL:
Official Citation:Wang Y, Xia J, Wang LV; Deep-tissue photoacoustic tomography of förster resonance energy transfer. J. Biomed. Opt. 0001;18(10):101316-101316. doi:10.1117/1.JBO.18.10.101316
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69238
Deposited By: Tony Diaz
Deposited On:27 Jul 2016 16:37
Last Modified:11 Nov 2021 04:11

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