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Heritability and Control of Differentiated Function in Cultured Cells

Davidson, E. H. (1963) Heritability and Control of Differentiated Function in Cultured Cells. Journal of General Physiology, 46 (5). pp. 983-998. ISSN 0022-1295. PMCID PMC2195304. doi:10.1085/jgp.46.5.983.

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An established tissue culture cell line which retains a differentiated function in vitro is described. The cell line is of connective tissue origin, and its characteristic property is the synthesis and secretion of acid mucopolysaccharides, mainly hyaluronic acid. This differentiated cell function, the activity of which depends on continuous gene action, was found to be possessed by each of eleven clonal substrains, and is therefore a genetically heritable cell character. Rate of acid mucopolysaccharide biosynthesis falls sharply under the influence of the environmental conditions existing in crowded cultures, and this rate also declines if protein synthesis is directly inhibited with puromycin. Environmental modification of a differentiated product of gene action is thus illustrated in this study.

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Additional Information:© 1963 Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license as described at and Submitted: 26 November 1962. Received for publication, November 26, 1962. Published May 1, 1963.
Issue or Number:5
PubMed Central ID:PMC2195304
Record Number:CaltechAUTHORS:20160727-101405513
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Official Citation:Heritability and Control of Differentiated Function in Cultured Cells E. H. Davidson J Gen Physiol 1963 46:983-998. Published May 1, 1963, doi:10.1085/jgp.46.5.983
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69245
Deposited By: Ruth Sustaita
Deposited On:27 Jul 2016 18:38
Last Modified:11 Nov 2021 04:11

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