CaltechAUTHORS
  A Caltech Library Service

Ultraviolet Sensitivity of the Biological Activity of ΦX174 Virus, Single-Stranded DNA, and RF DNA

Yarus, Michael and Sinsheimer, Robert L. (1967) Ultraviolet Sensitivity of the Biological Activity of ΦX174 Virus, Single-Stranded DNA, and RF DNA. Biophysical Journal, 7 (3). pp. 267-278. ISSN 0006-3495. PMCID PMC1368031. http://resolver.caltech.edu/CaltechAUTHORS:20160727-102724849

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20160727-102724849

Abstract

Action spectra for inactivation of ϕX virus, free ϕX single-stranded DNA, and double-stranded ϕX DNA (RF) have been measured using light of wavelength 225-302 mμ. The sensitivity of RF has been determined using bacterial hosts both capable and incapable of reactivation of UV damage. The inactivation of ϕX virus is due, at all wavelengths, to damage to its DNA; it appears that, below 240 mμ, energy absorbed by viral structural protein may inactivate the viral DNA. The variation of the probability of inactivation by an absorbed quantum (quantum yield) with wavelength, in the case of free-single-stranded ϕX DNA, suggests that energy absorbed by pyrimidine residues is more likely to yield inactivation than absorption by purines. This implies that energy transfer is not so extensive as to make all absorbed energy available to pyrimidines.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0006-3495(67)86587-1DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1368031/PubMed CentralArticle
Additional Information:© 1967 Biophysical Society. Received for publication 9 November 1966. This work is drawn from a thesis by M. Yarus submitted in partial fulfillment of the requirements for the Ph. D. degree. We would like to thank Max Delbruck for the use of the monochromator, which he and his group had calibrated and made usable. This work was supported, in part, by USPHS Fellowship 1F1 GM21,328-01 and by grants RG 6965 and GM 13554 from the U. S. Public Health Service.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)1F1 GM21, 328-01
NIHRG 6965
U.S. Public Health Service (USPHS)GM 13554
PubMed Central ID:PMC1368031
Record Number:CaltechAUTHORS:20160727-102724849
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160727-102724849
Official Citation:Michael Yarus, Robert L. Sinsheimer, Ultraviolet Sensitivity of the Biological Activity of ΦX174 Virus, Single-Stranded DNA, and RF DNA, Biophysical Journal, Volume 7, Issue 3, 1967, Pages 267-278, ISSN 0006-3495, http://dx.doi.org/10.1016/S0006-3495(67)86587-1. (http://www.sciencedirect.com/science/article/pii/S0006349567865871)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69248
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:27 Jul 2016 18:33
Last Modified:27 Jul 2016 18:33

Repository Staff Only: item control page